血管基质片段辅助游离脂肪移植早期移植物组织学变化及相关实验研究_《中国修复重建外科杂志》

作者：

袁艺 , 鲁峰 , 高建华

南方医科大学南方医院整形外科（广州，510515）;

关键词：

血管基质片段游离脂肪移植血管生成生长因子裸鼠

DOI：

10.7507/1002-1892.20130103

视频：

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目的探讨血管基质片段（vascular stromal fraction，SVF）辅助游离脂肪移植促进其早期存活的相关机制。方法取5例行腹部抽脂手术女性患者自愿捐赠的脂肪组织分离、培养SVF。4～6周龄裸鼠24只（体重15～18 g），将裸鼠背部左、右侧随机分为A、B组（n=24），采用Coleman技术分别于A、B组裸鼠背部皮下移植脂肪0.5 mL和含5 × 105个SVF的脂肪0.5 mL。移植后1、3、5、7 d分别取6只裸鼠，取出移植脂肪组织，行大体观察、CD31免疫组织化学染色及Western blot检测分析。结果随着移植时间增加，A、B组移植脂肪组织湿重均呈上升趋势，但各时间点A、B组间比较差异均无统计学意义（P gt; 0.05）。移植后1、3 d两组均未见CD31阳性细胞；5、7 d B组CD31阳性细胞数显著高于A组（P lt; 0.05），两组7 d时CD31阳性细胞数显著高于5 d时（P lt; 0.05）。Western blot检测示，两组移植脂肪中VEGF蛋白表达在移植后第3天达峰值，随后开始下降；1、3、5 d B组VEGF蛋白表达明显高于A组（P lt; 0.05）。两组移植脂肪中HGF蛋白表达在移植后前5 d均保持在较高水平，7 d开始下降，B组均明显高于A组（P lt; 0.05）。结论在游离脂肪移植早期，SVF通过旁分泌生长因子促进移植脂肪血管生成。

引用本文： 袁艺,鲁峰,高建华. 血管基质片段辅助游离脂肪移植早期移植物组织学变化及相关实验研究. 中国修复重建外科杂志, 2013, 27(4): 449-453. doi: 10.7507/1002-1892.20130103 复制

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3.	Miyagawa S, Sawa Y, Fukuda K, et al. Angiogenic gene cell therapy using suicide gene system regulates the effect of angiogenesis in infarcted rat heart. Transplantation, 2006, 81(6): 902-907.
4.	Taniyama Y, Morishita R, Aoki M, et al. Therapeutic angiogenesis induced by human hepatocyte growth factor gene in rat and rabbit hindlimb ischemia models: preclinical study for treatment of peripheral arterial disease. Gene Ther, 2001, 8(3): 181-189.
5.	Yoshimura K, Shigeura T, Matsumoto D, et al. Characterization of freshly isolated and cultured cells derived from the fatty and fluid portions of liposuction aspirates. J Cell Physiol, 2006, 208(1): 64-76.
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7.	Yoshimura K, Sato K, Aoi N, et al. Cell-assisted lipotransfer for facial lipoatrophy: efficacy of clinical use of adipose-derived stem cells. Dermatol Surg, 2008, 34(9): 1178-1185.
8.	Zuk PA, Zhu M, Ashjian P, et al. Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell, 2002, 13(12): 4279-4295.
9.	Tallone T, Realini C, Bohmler A, et al. Adult human adipose tissue contains several types of multipotent cells. J Cardiovasc Transl Res, 2011, 4(2): 200-210.
10.	Wilson A, Butler PE, Seifalian AM. Adipose-derived stem cells for clinical applications: a review. Cell Prolif, 2011, 44(1): 86-98.
11.	Shiffman MA, Mirrafati S. Fat transfer techniques: the effect of harvest and transfer methods on adipocyte viability and review of the literature. Dermatol Surg, 2001, 27(9): 819-826.
12.	Christiaens V, Lijnen HR. Angiogenesis and development of adipose tissue. Mol Cell Endocrinol, 2010, 318(1-2): 2-9.
13.	Yoshimura K, Suga H, Eto H. Adipose-derived stem/progenitor cells: roles in adipose tissue remodeling and potential use for soft tissue augmentation. Regen Med, 2009, 4(2): 265-273.
14.	Lee EY, Xia Y, Kim WS, et al. Hypoxia-enhanced wound-healing function of adipose-derived stem cells: increase in stem cell proliferation and up-regulation of VEGF and bFGF. Wound Repair Regen, 2009, 17(4): 540-547.
15.	Rubina K, Kalinina N, Efimenko A, et al. Adipose stromal cells stimulate angiogenesis via promoting progenitor cell differentiation, secretion of angiogenic factors, and enhancing vessel maturation. Tissue Eng Part A, 2009, 15(8): 2039-2050.
16.	Eto H, Kato H, Suga H, et al. The fate of adipocytes after nonvascularized fat grafting: evidence of early death and replacement of adipocytes. Plast Reconstr Surg, 2012, 129(5): 1081-1092.
17.	Maumus M, Peyrafitte JA, D’Angelo R, et al. Native human adipose stromal cells: localization, morphology and phenotype. Int J Obes (Lond), 2011, 35(9): 1141-1153.
18.	Traktuev DO, Prater DN, Merfeld-Clauss S, et al. Robust functional vascular network formation in vivo by cooperation of adipose progenitor and endothelial cells. Circ Res, 2009, 104(12): 1410-1420.
19.	Butala P, Hazen A, Szpalski C, et al. Endogenous stem cell therapy enhances fat graft survival. Plast Reconstr Surg, 2012, 130(2): 293-306.

1. Risau W. Mechanisms of angiogenesis. Nature, 1997, 386(6626): 671-674.
2. Yamaguchi T, Sawa Y, Miyamoto Y, et al. Therapeutic angiogenesis induced by injecting hepatocyte growth factor in ischemic canine hearts. Surg Today, 2005, 35(10): 855-860.
3. Miyagawa S, Sawa Y, Fukuda K, et al. Angiogenic gene cell therapy using suicide gene system regulates the effect of angiogenesis in infarcted rat heart. Transplantation, 2006, 81(6): 902-907.
4. Taniyama Y, Morishita R, Aoki M, et al. Therapeutic angiogenesis induced by human hepatocyte growth factor gene in rat and rabbit hindlimb ischemia models: preclinical study for treatment of peripheral arterial disease. Gene Ther, 2001, 8(3): 181-189.
5. Yoshimura K, Shigeura T, Matsumoto D, et al. Characterization of freshly isolated and cultured cells derived from the fatty and fluid portions of liposuction aspirates. J Cell Physiol, 2006, 208(1): 64-76.
6. Matsumoto D, Sato K, Gonda K, et al. Cell-assisted lipotransfer: Supportive use of human adipose-derived cells for soft tissue augmentation with lipoinjection. Tissue Eng, 2006, 12(12): 3375-3382.
7. Yoshimura K, Sato K, Aoi N, et al. Cell-assisted lipotransfer for facial lipoatrophy: efficacy of clinical use of adipose-derived stem cells. Dermatol Surg, 2008, 34(9): 1178-1185.
8. Zuk PA, Zhu M, Ashjian P, et al. Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell, 2002, 13(12): 4279-4295.
9. Tallone T, Realini C, Bohmler A, et al. Adult human adipose tissue contains several types of multipotent cells. J Cardiovasc Transl Res, 2011, 4(2): 200-210.
10. Wilson A, Butler PE, Seifalian AM. Adipose-derived stem cells for clinical applications: a review. Cell Prolif, 2011, 44(1): 86-98.
11. Shiffman MA, Mirrafati S. Fat transfer techniques: the effect of harvest and transfer methods on adipocyte viability and review of the literature. Dermatol Surg, 2001, 27(9): 819-826.
12. Christiaens V, Lijnen HR. Angiogenesis and development of adipose tissue. Mol Cell Endocrinol, 2010, 318(1-2): 2-9.
13. Yoshimura K, Suga H, Eto H. Adipose-derived stem/progenitor cells: roles in adipose tissue remodeling and potential use for soft tissue augmentation. Regen Med, 2009, 4(2): 265-273.
14. Lee EY, Xia Y, Kim WS, et al. Hypoxia-enhanced wound-healing function of adipose-derived stem cells: increase in stem cell proliferation and up-regulation of VEGF and bFGF. Wound Repair Regen, 2009, 17(4): 540-547.
15. Rubina K, Kalinina N, Efimenko A, et al. Adipose stromal cells stimulate angiogenesis via promoting progenitor cell differentiation, secretion of angiogenic factors, and enhancing vessel maturation. Tissue Eng Part A, 2009, 15(8): 2039-2050.
16. Eto H, Kato H, Suga H, et al. The fate of adipocytes after nonvascularized fat grafting: evidence of early death and replacement of adipocytes. Plast Reconstr Surg, 2012, 129(5): 1081-1092.
17. Maumus M, Peyrafitte JA, D’Angelo R, et al. Native human adipose stromal cells: localization, morphology and phenotype. Int J Obes (Lond), 2011, 35(9): 1141-1153.
18. Traktuev DO, Prater DN, Merfeld-Clauss S, et al. Robust functional vascular network formation in vivo by cooperation of adipose progenitor and endothelial cells. Circ Res, 2009, 104(12): 1410-1420.
19. Butala P, Hazen A, Szpalski C, et al. Endogenous stem cell therapy enhances fat graft survival. Plast Reconstr Surg, 2012, 130(2): 293-306.

《中国修复重建外科杂志》

血管基质片段辅助游离脂肪移植早期移植物组织学变化及相关实验研究

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《中国修复重建外科杂志》

血管基质片段辅助游离脂肪移植早期移植物组织学变化及相关实验研究

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