氢气饱和生理盐水对腹主动脉瘤大鼠主动脉残余应力的影响_《中国修复重建外科杂志》

作者：

宋玉祥 ¹ , 陈峰 ¹ , 熊江 ¹ , 郭伟 ¹ , 潘秀颉 ² , 贾森皓 ¹ , 刘杰 ¹

1解放军总医院血管外科（北京，100853）;;
2 军事医学科学院放射与辐射医学研究所;

关键词：

氢气饱和生理盐水腹主动脉瘤张开角残余应力血管重建大鼠

DOI：

10.7507/1002-1892.20130193

视频：

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目的通过观察腹主动脉瘤（abnormal aortic aneurysm，AAA）大鼠腹主动脉直径、扩张率、血管壁厚度及张开角等指标的变化，了解氢气饱和生理盐水对AAA大鼠主动脉残余应力的影响，探讨其对AAA形成的抑制作用。方法选取健康雄性SD大鼠20只（体重200～220 g），采用肾下腹主动脉CaCl2孵育法制备大鼠AAA模型，随机分为实验组和对照组，每组10只。建模后当天开始，实验组于腹腔注射氢气饱和生理盐水（5 mL/kg），对照组注射生理盐水（5 mL/ kg）；每天1次，共28 d。建模后28 d处死两组动物，测量腹主动脉直径并计算扩张率，选取瘤体位置动脉环测量腹主动脉张开角及血管壁厚度，并对标本行HE染色和醛品红弹力纤维特殊染色观察主动脉壁炎性细胞浸润及中膜弹力纤维破坏情况。结果建模后28 d，实验组与对照组腹主动脉直径均较术前显著增加（P lt; 0.01）；扩张率分别为65% ± 15%和128% ± 54%，实验组显著低于对照组（t=3.611，P=0.005）。实验组与对照组腹主动脉张开角分别为（88.78 ± 29.20）°和（44.23 ± 28.52）°，血管壁厚度分别为（0.14 ± 0.03）mm和（0.36 ± 0.05）mm，两组比较差异均有统计学意义（P lt; 0.01）。组织学观察示，实验组主动脉壁存在少量炎性细胞浸润，弹力纤维未见明显受损；对照组主动脉壁炎性细胞浸润明显，弹力纤维断裂受损严重。结论氢气饱和生理盐水通过增大主动脉的残余应力、减轻血管壁的组织重建程度，来维持腹主动脉更好的力学特性，减少腹主动脉扩张，抑制AAA的形成。

引用本文： 宋玉祥,陈峰,熊江,郭伟,潘秀颉,贾森皓,刘杰. 氢气饱和生理盐水对腹主动脉瘤大鼠主动脉残余应力的影响. 中国修复重建外科杂志, 2013, 27(7): 881-884. doi: 10.7507/1002-1892.20130193 复制

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4.	Shen L, Wang J, Liu K, et al. Hydrogen-rich saline is cerebroprotective in a rat model of deep hypothermic circulatory arrest. Neurochem Res, 2011, 36(8): 1501-1511.
5.	Kajiya M, Silva MJ, Sato K, et al. Hydrogen mediates suppression of colon inflammation induced by dextran sodium sulfate. Biochem Biophys Res Commun, 2009, 386(1): 11-15.
6.	Isenburg JC, Simionescu DT, Starcher BC, et al. Elastin stabilization for treatment of abdominal aortic aneurysms. Circulation, 2007, 115(13): 1729-1737.
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12.	Chiou AC, Chiu B, Pearce WH. Murine aortic aneurysm produced by periarterial application of calcium chloride. J Surg Res, 2001, 99(2): 371-376.
13.	Cardinal JS, Zhan J, Wang Y, et al. Oral hydrogen water prevents chronic allograft nephropathy in rats. Kidney Int, 2010, 77(2): 101-109.
14.	Buchholz BM, Kaczorowski DJ, Sugimoto R, et al. Hydrogen inhalation ameliorates oxidative stress in transplantation induced intestinal graft injury. Am J Transplant, 2008, 8(10): 2015-2024.
15.	Ohta S. Recent progress toward hydrogen medicine: potential of molecular hydrogen for preventive and therapeutic applications. Curr Pharm Des, 2011, 17(22): 2241-2252.

1. White JJ, Dalman RL. Gaining new insights into early abdominal aortic aneurysm disease. Perm J, 2008, 12(2): 10-14.
2. Fung YC. What are the residual stresses doing in our blood vessels? Ann Biomed Eng, 1991, 19(3): 237-249.
3. Ohsawa I, Nishimaki K, Yamagata K, et al. Consumption of hydrogen water prevents atherosclerosis in apolipoprotein E knockout mice. Biochem Biophys Res Commun, 2008, 377(4): 1195-1198.
4. Shen L, Wang J, Liu K, et al. Hydrogen-rich saline is cerebroprotective in a rat model of deep hypothermic circulatory arrest. Neurochem Res, 2011, 36(8): 1501-1511.
5. Kajiya M, Silva MJ, Sato K, et al. Hydrogen mediates suppression of colon inflammation induced by dextran sodium sulfate. Biochem Biophys Res Commun, 2009, 386(1): 11-15.
6. Isenburg JC, Simionescu DT, Starcher BC, et al. Elastin stabilization for treatment of abdominal aortic aneurysms. Circulation, 2007, 115(13): 1729-1737.
7. 吴晓飞, 张健, 辛世杰, 等. 雌激素在大鼠腹主动脉瘤形成中的作用. 中华医学杂志, 2007, 87(7): 471-474.
8. Fung YC, Liu SQ. Changes of zero-stress state of rat pulmonary arteries in hypoxic hypertension. J Appl Physiol, 1991, 70(6): 2455-2470.
9. Liu SQ, Fung YC. Relationship between hypertension, hypertrophy, and opening angle of zero-stress state of arteries following aortic constriction. J Biomech Eng, 1989, 111(4): 325-335.
10. Zhao J, Day J, Yuan ZF, et al. Regional arterial stress-strain distributions referenced to the zero-stress state in the rat. Am J Physiol Heart Circ Physiol, 2002, 282(2): H622-629.
11. 卢晓, 王公瑞, 叶志义, 等. 主动脉血管壁三种主要组份对其残余应力贡献的分析. 中国生物医学工程学报, 2001, 20(5): 445-450, 472.
12. Chiou AC, Chiu B, Pearce WH. Murine aortic aneurysm produced by periarterial application of calcium chloride. J Surg Res, 2001, 99(2): 371-376.
13. Cardinal JS, Zhan J, Wang Y, et al. Oral hydrogen water prevents chronic allograft nephropathy in rats. Kidney Int, 2010, 77(2): 101-109.
14. Buchholz BM, Kaczorowski DJ, Sugimoto R, et al. Hydrogen inhalation ameliorates oxidative stress in transplantation induced intestinal graft injury. Am J Transplant, 2008, 8(10): 2015-2024.
15. Ohta S. Recent progress toward hydrogen medicine: potential of molecular hydrogen for preventive and therapeutic applications. Curr Pharm Des, 2011, 17(22): 2241-2252.

《中国修复重建外科杂志》

氢气饱和生理盐水对腹主动脉瘤大鼠主动脉残余应力的影响

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《中国修复重建外科杂志》

氢气饱和生理盐水对腹主动脉瘤大鼠主动脉残余应力的影响

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