• 1. 第三軍醫(yī)大學(xué)附屬西南醫(yī)院普外科( 重慶 400038) ;;
  • 2. 河北省邢臺市第五人民醫(yī)院內(nèi)科(邢臺054027) ;;
  • 3. 解放軍南京軍區(qū)180 醫(yī)院普外科(福建泉州362000);

目的　觀察T 淋巴細(xì)胞瘤侵襲轉(zhuǎn)移誘導(dǎo)因子1 ( Tiam 1) 反義寡核苷酸(ASODN) 轉(zhuǎn)染對胃癌細(xì)胞形體重塑的影響。方法　采用層粘連蛋白黏附法,由胃癌MKN245 細(xì)胞株(M0 ) 中篩選獲得高侵襲轉(zhuǎn)移亞株(MH ) 。以脂質(zhì)體介導(dǎo)將Tiam 1 ASODN 轉(zhuǎn)染至MH 細(xì)胞中,并應(yīng)用逆轉(zhuǎn)錄聚合酶鏈反應(yīng)(RT2PCR) 及流式細(xì)胞技術(shù)分別檢測Tiam 1 mRNA 和蛋白的表達(dá)。采用HE 染色、細(xì)胞骨架蛋白染色及掃描電鏡技術(shù)觀察轉(zhuǎn)染與未轉(zhuǎn)染MH 細(xì)胞在形態(tài)學(xué)方面的不同。結(jié)果　與未轉(zhuǎn)染組相比,應(yīng)用0. 43μmol/ L Tiam 1 ASODN 轉(zhuǎn)染可特異性抑制胃癌MH細(xì)胞中Tiam 1 mRNA 和蛋白的表達(dá)( P lt; 0. 01) ; 轉(zhuǎn)染MH 細(xì)胞的膜表面突起及偽足變稀疏或縮短,細(xì)胞骨架結(jié)構(gòu)紊亂程度降低,斑點(diǎn)狀肌動(dòng)蛋白小體減少。結(jié)論　特異性ASODN 轉(zhuǎn)染可有效抑制Tiam 1 在胃癌細(xì)胞中的表達(dá)及胃癌細(xì)胞形體重塑,這可能對胃癌細(xì)胞的侵襲轉(zhuǎn)移產(chǎn)生影響。

引用本文： 朱金明,余佩武,李翠芳,吳淼,趙永亮. 反義抑制Tiam 1 對胃癌細(xì)胞形體重塑影響的實(shí)驗(yàn)研究. 中國普外基礎(chǔ)與臨床雜志, 2008, 15(1): 12-16. doi: 復(fù)制

1. <><> [1]Jaeken L.A new list of functions of the cytoskeleton[J].IUBMB Life,2007; 59(3)∶127.
2. Minard ME,Ellis LM,Gallick GE.Tiam 1 regulates cell adhesion,migration and apoptosis in colon tumor cells[J].Clin Exp Metastasis,2006; 23(5-6)∶301.
3. 朱金明,余佩武.Tiam 1表達(dá)與胃癌侵襲轉(zhuǎn)移關(guān)系的臨床和實(shí)驗(yàn)研究[J].臨床與實(shí)驗(yàn)病理學(xué)雜志,2006; 22(1)∶85.
4. 王舒寶,王俊.胃癌復(fù)發(fā)與轉(zhuǎn)移的有關(guān)問題及綜合治療[J].中國普外基礎(chǔ)與臨床雜志,2006; 13(1)∶9.
5. 陳向榮,任衛(wèi)平,童菊芳,等.粘附法篩選胃癌細(xì)胞亞株[J].胃腸病學(xué),2000; 5(2)∶116.
6. 張進(jìn)華,丁彥青,楊學(xué)皆.介紹一種細(xì)胞骨架蛋白改良染色法[J].診斷病理學(xué)雜志,1996; 3(4)∶235.
7. Mertens AE,Roovers RC,Collard JG.Regulation of Tiam 1-Rac signalling[J].FEBS Lett,2003; 546(1)∶11.
8. Strumane K,Rygiel TP,Collard JG.The rac activator tiam 1 and ras-induced oncogenesis[J].Methods Enzymol,2005; 407∶269.
9. Yamauchi J,Miyamoto Y,Tanoue A,et al.Ras activation of a Rac 1 exchange factor,Tiam 1,mediates neurotrophin-3-induced Schwann cell migration[J].Proc Natl Acad Sci USA,2005; 102(41)∶14889.
10. Liu L,Zhao L,Zhang Y,et al.Proteomic analysis of Tiam 1-mediated metastasis in colorectal cancer[J].Cell Biol Int,2007; 31(8)∶805.
11. Engers R,Mueller M,Walter A,et al.Prognostic relevance of Tiam 1 protein expression in prostate carcinomas[J].Br J Cancer,2006; 95(8)∶1081.
12. Minard ME,Kim LS,Price JE,et al.The role of the guanine nucleotide exchange factor Tiam 1 in cellular migration,invasion,adhesion and tumor progression[J].Breast Cancer Res Treat,2004; 84(1)∶21.
13. Pawlak G,Helfman DM.Cytoskeletal changes in cell transformation and tumorigenesis[J].Curr Opin Genet Dev,2001; 11(1)∶41.
14. Engers R,Gabbert HE.Mechanisms of tumor metastasis:cell biological aspects and clinical implications[J].J Cancer Res Clin Oncol,2000; 126(12)∶682.
15. Lleonart ME,Martin-Duque P,Sanchez-Prieto R,et al.Tum-or heterogeneity:morphological,molecular and clinical implications[J].Histol Histopathol,2000; 15(3)∶881.
16. Hoenger A,Nicastro D.Electron microscopy of microtubule-ba-sed cytoskeletal machinery[J].Methods Cell Biol,2007; 79∶437.
17. Lenormand G,Fredberg JJ.Deformability,dynamics,and remodeling of cytoskeleton of the adherent living cell[J].Biorheology,2006; 43(1)∶1.
18. Keely PJ,Westwick JK,Whitehead IP,et al.Cdc42 and Rac 1 induce integrin-mediated cell motility and invasiveness through PI(3)K[J].Nature,1997; 390(6660)∶632.
19. Delon I,Brown NH.Integrins and the actin cytoskeleton[J].Curr Opin Cell Biol,2007; 19(1)∶43.
20. Bourguignon LY,Zhu H,Shao L,et al.CD44 interaction with tiam 1 promotes Rac 1 signaling and hyaluronic acid-mediated breast tumor cell migration[J].J Biol Chem,2000; 275(3)∶1829.
21. 徐勝軍,胡炳奎,凌志強(qiáng),等.CD44v6在胃癌中的表達(dá)及其臨床意義[J].中國普外基礎(chǔ)與臨床雜志,2006; 13(1)∶42.
  1. 1. <><> [1]Jaeken L.A new list of functions of the cytoskeleton[J].IUBMB Life,2007; 59(3)∶127.
  2. 2. Minard ME,Ellis LM,Gallick GE.Tiam 1 regulates cell adhesion,migration and apoptosis in colon tumor cells[J].Clin Exp Metastasis,2006; 23(5-6)∶301.
  3. 3. 朱金明,余佩武.Tiam 1表達(dá)與胃癌侵襲轉(zhuǎn)移關(guān)系的臨床和實(shí)驗(yàn)研究[J].臨床與實(shí)驗(yàn)病理學(xué)雜志,2006; 22(1)∶85.
  4. 4. 王舒寶,王俊.胃癌復(fù)發(fā)與轉(zhuǎn)移的有關(guān)問題及綜合治療[J].中國普外基礎(chǔ)與臨床雜志,2006; 13(1)∶9.
  5. 5. 陳向榮,任衛(wèi)平,童菊芳,等.粘附法篩選胃癌細(xì)胞亞株[J].胃腸病學(xué),2000; 5(2)∶116.
  6. 6. 張進(jìn)華,丁彥青,楊學(xué)皆.介紹一種細(xì)胞骨架蛋白改良染色法[J].診斷病理學(xué)雜志,1996; 3(4)∶235.
  7. 7. Mertens AE,Roovers RC,Collard JG.Regulation of Tiam 1-Rac signalling[J].FEBS Lett,2003; 546(1)∶11.
  8. 8. Strumane K,Rygiel TP,Collard JG.The rac activator tiam 1 and ras-induced oncogenesis[J].Methods Enzymol,2005; 407∶269.
  9. 9. Yamauchi J,Miyamoto Y,Tanoue A,et al.Ras activation of a Rac 1 exchange factor,Tiam 1,mediates neurotrophin-3-induced Schwann cell migration[J].Proc Natl Acad Sci USA,2005; 102(41)∶14889.
  10. 10. Liu L,Zhao L,Zhang Y,et al.Proteomic analysis of Tiam 1-mediated metastasis in colorectal cancer[J].Cell Biol Int,2007; 31(8)∶805.
  11. 11. Engers R,Mueller M,Walter A,et al.Prognostic relevance of Tiam 1 protein expression in prostate carcinomas[J].Br J Cancer,2006; 95(8)∶1081.
  12. 12. Minard ME,Kim LS,Price JE,et al.The role of the guanine nucleotide exchange factor Tiam 1 in cellular migration,invasion,adhesion and tumor progression[J].Breast Cancer Res Treat,2004; 84(1)∶21.
  13. 13. Pawlak G,Helfman DM.Cytoskeletal changes in cell transformation and tumorigenesis[J].Curr Opin Genet Dev,2001; 11(1)∶41.
  14. 14. Engers R,Gabbert HE.Mechanisms of tumor metastasis:cell biological aspects and clinical implications[J].J Cancer Res Clin Oncol,2000; 126(12)∶682.
  15. 15. Lleonart ME,Martin-Duque P,Sanchez-Prieto R,et al.Tum-or heterogeneity:morphological,molecular and clinical implications[J].Histol Histopathol,2000; 15(3)∶881.
  16. 16. Hoenger A,Nicastro D.Electron microscopy of microtubule-ba-sed cytoskeletal machinery[J].Methods Cell Biol,2007; 79∶437.
  17. 17. Lenormand G,Fredberg JJ.Deformability,dynamics,and remodeling of cytoskeleton of the adherent living cell[J].Biorheology,2006; 43(1)∶1.
  18. 18. Keely PJ,Westwick JK,Whitehead IP,et al.Cdc42 and Rac 1 induce integrin-mediated cell motility and invasiveness through PI(3)K[J].Nature,1997; 390(6660)∶632.
  19. 19. Delon I,Brown NH.Integrins and the actin cytoskeleton[J].Curr Opin Cell Biol,2007; 19(1)∶43.
  20. 20. Bourguignon LY,Zhu H,Shao L,et al.CD44 interaction with tiam 1 promotes Rac 1 signaling and hyaluronic acid-mediated breast tumor cell migration[J].J Biol Chem,2000; 275(3)∶1829.
  21. 21. 徐勝軍,胡炳奎,凌志強(qiáng),等.CD44v6在胃癌中的表達(dá)及其臨床意義[J].中國普外基礎(chǔ)與臨床雜志,2006; 13(1)∶42.