• 福建醫(yī)科大學(xué)教學(xué)醫(yī)院福建省腫瘤醫(yī)院 (福州350014);

目的 　綜述熱休克蛋白70（heat shock protein 70，HSP70）疫苗在消化道腫瘤中的研究進(jìn)展。
方法 　對近年來的相關(guān)文獻(xiàn)進(jìn)行分析。
結(jié)果 　HSP70能與腫瘤特異性抗原結(jié)合，形成HSP70-多肽復(fù)合物； 它作為“分子伴侶”參與腫瘤免疫作用，激活機(jī)體特異和非特異免疫反應(yīng)。
結(jié)論 　HSP70的應(yīng)用在消化道腫瘤的預(yù)防和治療中顯示出誘人的前景。

引用本文： 劉勝,應(yīng)敏剛. 熱休克蛋白70疫苗在消化道腫瘤研究中的進(jìn)展. 中國普外基礎(chǔ)與臨床雜志, 2007, 14(6): 728-730. doi: 復(fù)制

1. 　Yokota S, Kitahara M, Nagata K. Benzylidene lactam compound, KNK437, a novel inhibitor of acquisition of thermotolerance and heat shock protein induction in human colon carcinoma cells [J]. Cancer Res, 2000; 60(11)∶2942.
2. 　Ozdemirler Erata G, Kanbaqli O, Durlanik O, et al. Induced oxidative stress and decreased expression of inducible heat shock protein 70 (ihsp 70) in patients with colorectal adenocarcinomas [J]. Jpn J Clin Oncol， 2005; 35(2)∶74.
3. 　Ueda G, Tamura Y, Hirai I, et al. Tumor-derived heat shock protein 70-pulsed dendritic cells elicit tumor-specific cytotoxic T lymphocytes (CTLs) and tumor immunity [J]. Cancer Sci， 2004; 95(3)∶248.
4. 　Maier T, Tun-Kyi A, Tassis A, et al. Vaccination of patients with cutaneous T-cell lymphoma using intranodal injection of autologous tumor-lysate-pulsed dendritic cells [J]. Blood， 2003; 102(7)∶2338.
5. 　Musch MW, Kaplan B, Chang EB. Role of increased basal expression of heat shock protein 72 in colonic epithelial c2BBE adenocarcinoma cells [J]. Cell Growth Differ， 2001; 12(8)∶419.
6. 　王小平, 劉國貞, 宋愛利, 等. 人肝癌組織熱休克蛋白70的表達(dá) [J]. 首都醫(yī)科大學(xué)學(xué)報, 2004; 25(2)∶186.
7. 　李春梅, 劉志峰, 張紅緒, 等. Hsp70和PCNA在NIH小鼠前胃癌變過程中的表達(dá)及意義 [J]. 細(xì)胞與分子免疫學(xué)雜志, 2004; 20(5)∶625.
8. 　Wang XP, Qiu FR, Liu GZ, et al. Correlation between clinicopathology and expression of heat shock protein 70 and glucose-regulated protein 94 in human colonic adenocarcinoma [J]. World J Gastroenterol， 2005; 11(7)∶1056.
9. 　Massé D, Ebstein F, Bougras G, et al. Increased expression of inducible HSP70 in apoptotic cells is correlated with their efficacy for antitumor vaccine therapy [J]. Int J Cancer， 2004; 111(4)∶575.
10. 　Massa C, Melani C, Colombo MP. Chaperon and adjuvant activity of hsp70: different natural killer requirement for cross-priming of chaperoned and bystander antigens [J]. Cancer Res， 2005; 65(17)∶7942.
11. 　Krause SW, Gastpar R, Andreesen R, et al. Treatment of colon and lung cancer patients with ex vivo heat shock protein 70-peptide-activated, autologous natural killer cells: a clinical phase Ⅰ trial [J]. Clin Cancer Res， 2004; 10(11)∶3699.
12. 　Wan T, Zhou X, Chen G, et al. Novel heat shock protein Hsp70L1 activates dendritic cells and acts as a Th1 polarizing adjuvant [J]. Blood， 2004; 103(5)∶1747.
13. 　史斌, 陳以娟, 王申五, 等. 熱休克蛋白-樹突狀細(xì)胞疫苗對慢性髓系白血病免疫治療作用的體外研究 [J]. 中華內(nèi)科雜志, 2004; 43(5)∶380.
14. 　錢世鹍, 閔軍, 褚忠華, 等. 熱休克蛋白對樹突狀細(xì)胞成熟的影響研究 [J]. 中國病理生理雜志, 2004; 20(7)∶1190.
15. 　萬敏, 張培因, 王宣軍, 等. 熱休克蛋白融合蛋白對人樹突狀細(xì)胞分化成熟的刺激作用 [J]. 吉林大學(xué)學(xué)報（醫(yī)學(xué)版）, 2004; 30(6)∶835.
16. 　Srivastava PK, MR Das. The serologically unique cell surface of zajdela ascitic hepatoma is also it tumor-associated transplantation antigen [J]. Int J Cancer, 1984; 33(3)∶417.
17. 　黃波, 馮作化, 張桂梅. Hsp70-腫瘤抗原肽復(fù)合物修飾的DC疫苗體內(nèi)外特異性抗瘤作用 [J]. 中國免疫學(xué)雜志, 2002; 18(9)∶614.
18. 　陳代雄, 蘇艷蓉, 錢振超. HSP70-腫瘤肽對小鼠肝癌的抗腫瘤免疫效應(yīng) [J]. 中國腫瘤生物治療雜志, 2002; 9(3)∶163.
19. 　袁慶文, 劉學(xué)政, 劉學(xué)農(nóng), 等. 胃癌Hsp70-肽復(fù)合物致敏樹突狀細(xì)胞抗腫瘤作用的研究 [J]. 江西醫(yī)學(xué)院學(xué)報, 2005; 45(3)∶1.
20. 　華贇鵬, 梁力建, 黃潔夫. 熱休克蛋白與肝缺血再灌注損傷 [J]. 中國普外基礎(chǔ)與臨床雜志, 2000; 7(4)∶269.
21. 　Todryk SM, Eaton J, Birchall L, et al. Heated tumour cells of autologous and allogeneic origin elicit anti-tumour immunity [J]. Cancer Immunol Immunother， 2004; 53(4)∶323.
22. 　Nylandsted J, Wick W, Hirt UA, et al. Eradication of glioblastoma, and breast and colon carcinoma xenografts by Hsp70 depletion [J]. Cancer Res， 2002; 62(24)∶7139.
23. 　邱健, 李國威, 隋延仿, 等. 熱休克腫瘤細(xì)胞抗原負(fù)載的樹突狀細(xì)胞瘤苗對原位接種結(jié)腸腫瘤的免疫預(yù)防作用 [J]. 世界華人消化雜志, 2005; 13(5)∶635.
  1. 1. 　Yokota S, Kitahara M, Nagata K. Benzylidene lactam compound, KNK437, a novel inhibitor of acquisition of thermotolerance and heat shock protein induction in human colon carcinoma cells [J]. Cancer Res, 2000; 60(11)∶2942.
  2. 2. 　Ozdemirler Erata G, Kanbaqli O, Durlanik O, et al. Induced oxidative stress and decreased expression of inducible heat shock protein 70 (ihsp 70) in patients with colorectal adenocarcinomas [J]. Jpn J Clin Oncol， 2005; 35(2)∶74.
  3. 3. 　Ueda G, Tamura Y, Hirai I, et al. Tumor-derived heat shock protein 70-pulsed dendritic cells elicit tumor-specific cytotoxic T lymphocytes (CTLs) and tumor immunity [J]. Cancer Sci， 2004; 95(3)∶248.
  4. 4. 　Maier T, Tun-Kyi A, Tassis A, et al. Vaccination of patients with cutaneous T-cell lymphoma using intranodal injection of autologous tumor-lysate-pulsed dendritic cells [J]. Blood， 2003; 102(7)∶2338.
  5. 5. 　Musch MW, Kaplan B, Chang EB. Role of increased basal expression of heat shock protein 72 in colonic epithelial c2BBE adenocarcinoma cells [J]. Cell Growth Differ， 2001; 12(8)∶419.
  6. 6. 　王小平, 劉國貞, 宋愛利, 等. 人肝癌組織熱休克蛋白70的表達(dá) [J]. 首都醫(yī)科大學(xué)學(xué)報, 2004; 25(2)∶186.
  7. 7. 　李春梅, 劉志峰, 張紅緒, 等. Hsp70和PCNA在NIH小鼠前胃癌變過程中的表達(dá)及意義 [J]. 細(xì)胞與分子免疫學(xué)雜志, 2004; 20(5)∶625.
  8. 8. 　Wang XP, Qiu FR, Liu GZ, et al. Correlation between clinicopathology and expression of heat shock protein 70 and glucose-regulated protein 94 in human colonic adenocarcinoma [J]. World J Gastroenterol， 2005; 11(7)∶1056.
  9. 9. 　Massé D, Ebstein F, Bougras G, et al. Increased expression of inducible HSP70 in apoptotic cells is correlated with their efficacy for antitumor vaccine therapy [J]. Int J Cancer， 2004; 111(4)∶575.
  10. 10. 　Massa C, Melani C, Colombo MP. Chaperon and adjuvant activity of hsp70: different natural killer requirement for cross-priming of chaperoned and bystander antigens [J]. Cancer Res， 2005; 65(17)∶7942.
  11. 11. 　Krause SW, Gastpar R, Andreesen R, et al. Treatment of colon and lung cancer patients with ex vivo heat shock protein 70-peptide-activated, autologous natural killer cells: a clinical phase Ⅰ trial [J]. Clin Cancer Res， 2004; 10(11)∶3699.
  12. 12. 　Wan T, Zhou X, Chen G, et al. Novel heat shock protein Hsp70L1 activates dendritic cells and acts as a Th1 polarizing adjuvant [J]. Blood， 2004; 103(5)∶1747.
  13. 13. 　史斌, 陳以娟, 王申五, 等. 熱休克蛋白-樹突狀細(xì)胞疫苗對慢性髓系白血病免疫治療作用的體外研究 [J]. 中華內(nèi)科雜志, 2004; 43(5)∶380.
  14. 14. 　錢世鹍, 閔軍, 褚忠華, 等. 熱休克蛋白對樹突狀細(xì)胞成熟的影響研究 [J]. 中國病理生理雜志, 2004; 20(7)∶1190.
  15. 15. 　萬敏, 張培因, 王宣軍, 等. 熱休克蛋白融合蛋白對人樹突狀細(xì)胞分化成熟的刺激作用 [J]. 吉林大學(xué)學(xué)報（醫(yī)學(xué)版）, 2004; 30(6)∶835.
  16. 16. 　Srivastava PK, MR Das. The serologically unique cell surface of zajdela ascitic hepatoma is also it tumor-associated transplantation antigen [J]. Int J Cancer, 1984; 33(3)∶417.
  17. 17. 　黃波, 馮作化, 張桂梅. Hsp70-腫瘤抗原肽復(fù)合物修飾的DC疫苗體內(nèi)外特異性抗瘤作用 [J]. 中國免疫學(xué)雜志, 2002; 18(9)∶614.
  18. 18. 　陳代雄, 蘇艷蓉, 錢振超. HSP70-腫瘤肽對小鼠肝癌的抗腫瘤免疫效應(yīng) [J]. 中國腫瘤生物治療雜志, 2002; 9(3)∶163.
  19. 19. 　袁慶文, 劉學(xué)政, 劉學(xué)農(nóng), 等. 胃癌Hsp70-肽復(fù)合物致敏樹突狀細(xì)胞抗腫瘤作用的研究 [J]. 江西醫(yī)學(xué)院學(xué)報, 2005; 45(3)∶1.
  20. 20. 　華贇鵬, 梁力建, 黃潔夫. 熱休克蛋白與肝缺血再灌注損傷 [J]. 中國普外基礎(chǔ)與臨床雜志, 2000; 7(4)∶269.
  21. 21. 　Todryk SM, Eaton J, Birchall L, et al. Heated tumour cells of autologous and allogeneic origin elicit anti-tumour immunity [J]. Cancer Immunol Immunother， 2004; 53(4)∶323.
  22. 22. 　Nylandsted J, Wick W, Hirt UA, et al. Eradication of glioblastoma, and breast and colon carcinoma xenografts by Hsp70 depletion [J]. Cancer Res， 2002; 62(24)∶7139.
  23. 23. 　邱健, 李國威, 隋延仿, 等. 熱休克腫瘤細(xì)胞抗原負(fù)載的樹突狀細(xì)胞瘤苗對原位接種結(jié)腸腫瘤的免疫預(yù)防作用 [J]. 世界華人消化雜志, 2005; 13(5)∶635.