• 1.成都軍區(qū)總醫(yī)院全軍普外中心（成都610083）;;
  • 2.廣州珠江醫(yī)院肝膽外科（廣州510282）;

目的 　研究原發(fā)性肝細(xì)胞癌發(fā)生、發(fā)展過程中肝組織增殖細(xì)胞核抗原（proliferating cell nuclear antigen, PCNA）的動態(tài)演變規(guī)律。
方法 　將60只SD大鼠按隨機(jī)數(shù)字表法隨機(jī)分為對照組（20只）和實(shí)驗(yàn)組（40只）。用化學(xué)致癌劑3,3′-二氨基聯(lián)苯胺（3，3′-diaminobenzidine,DAB）誘發(fā)SD大鼠肝癌，采用免疫組織化學(xué)方法連續(xù)監(jiān)測各時(shí)相肝組織中PCNA的表達(dá)，光鏡下觀察肝組織的病理變化。
結(jié)果 　本實(shí)驗(yàn)的誘癌過程可分為炎性變期、增生纖維化期和癌變期3個(gè)階段； PCNA的表達(dá)最早出現(xiàn)于炎性變早期匯管區(qū)的卵圓細(xì)胞中，并在匯管區(qū)呈區(qū)域性過表達(dá)分布； 炎性變中期，PCNA表達(dá)陽性率達(dá)高峰，隨后下降，在癌變期又升高，在整個(gè)病理演變過程中其表達(dá)具波動性。
結(jié)論 　在致癌因素的存在下，PCNA首先表達(dá)于卵圓細(xì)胞； PCNA表達(dá)的動態(tài)演變對肝癌的發(fā)生有一定的預(yù)警作用。

引用本文： 龔加慶,方馳華. 大鼠肝癌發(fā)生發(fā)展過程中肝組織增殖細(xì)胞核抗原動態(tài)演變規(guī)律. 中國普外基礎(chǔ)與臨床雜志, 2007, 14(1): 11-14. doi: 復(fù)制

1. 　Mattern J, Koomagi R, Volm M. Biological characterization of subgroups of squamous cell lung carcinomas [J].Clin Cancer Res, 1999; 5(6)∶1459.
2. 　Chung JK, Lee T, Jung IM, et al. Expression of cell cycle regulators during smooth muscle cell proliferation after balloon catheter injury of rat artery [J]. J Korean Med Sci, 2004; 19(3)∶327.
3. 　Guettier C. Which stem cells for adult liver? [J].Ann Pathol, 2005; 25(1)∶33.
4. 　Oh BK, Lee CH, Park C, et al. Telomerase regulation and progressive telomere shortening of rat hepatic stemlike epithelial cells during in vitro aging [J]. Exp Cell Res, 2004; 298(2)∶445.
5. 　Hatch HM, Zheng D, Jorgensen ML, et al. SDF1alpha/CXCR4: a mechanism for hepatic oval cell activation and bone marrow stem cell recruitment to the injured liver of rats [J]. Cloning Stem Cells, 2002; 4(4)∶339.
6. 　龔加慶， 方馳華， 李雅. 肝卵圓細(xì)胞在進(jìn)行性肝損傷過程中分布及遷移的實(shí)驗(yàn)研究 [J]. 中國普外基礎(chǔ)與臨床雜志, 2004; 11(5)∶389.
7. 　韋偉， 龔建平， 裘法祖. 正常和異常肝組織增生中細(xì)胞增殖與凋亡的關(guān)系 [J]. 第三軍醫(yī)大學(xué)學(xué)報(bào), 2001； 18（2）∶156.
8. 　Fiegel HC, Gluer S, Roth B, et al. Stemlike cells in human hepatoblastoma [J]. J Histochem Cytochem, 2004; 52(11)∶1495.
  1. 1. 　Mattern J, Koomagi R, Volm M. Biological characterization of subgroups of squamous cell lung carcinomas [J].Clin Cancer Res, 1999; 5(6)∶1459.
  2. 2. 　Chung JK, Lee T, Jung IM, et al. Expression of cell cycle regulators during smooth muscle cell proliferation after balloon catheter injury of rat artery [J]. J Korean Med Sci, 2004; 19(3)∶327.
  3. 3. 　Guettier C. Which stem cells for adult liver? [J].Ann Pathol, 2005; 25(1)∶33.
  4. 4. 　Oh BK, Lee CH, Park C, et al. Telomerase regulation and progressive telomere shortening of rat hepatic stemlike epithelial cells during in vitro aging [J]. Exp Cell Res, 2004; 298(2)∶445.
  5. 5. 　Hatch HM, Zheng D, Jorgensen ML, et al. SDF1alpha/CXCR4: a mechanism for hepatic oval cell activation and bone marrow stem cell recruitment to the injured liver of rats [J]. Cloning Stem Cells, 2002; 4(4)∶339.
  6. 6. 　龔加慶， 方馳華， 李雅. 肝卵圓細(xì)胞在進(jìn)行性肝損傷過程中分布及遷移的實(shí)驗(yàn)研究 [J]. 中國普外基礎(chǔ)與臨床雜志, 2004; 11(5)∶389.
  7. 7. 　韋偉， 龔建平， 裘法祖. 正常和異常肝組織增生中細(xì)胞增殖與凋亡的關(guān)系 [J]. 第三軍醫(yī)大學(xué)學(xué)報(bào), 2001； 18（2）∶156.
  8. 8. 　Fiegel HC, Gluer S, Roth B, et al. Stemlike cells in human hepatoblastoma [J]. J Histochem Cytochem, 2004; 52(11)∶1495.