• 1.華中科技大學(xué)同濟(jì)醫(yī)學(xué)院附屬協(xié)和醫(yī)院胃腸外科中心（武漢430022）;;
  • 2.湖北省黃岡市第一人民醫(yī)院普外科（黃岡438000）;;
  • 3.華中科技大學(xué)同濟(jì)醫(yī)學(xué)院病原生物學(xué)教研室（武漢430030）;;
  • 通訊作者： 黃文廣;

【摘要】目的探討干擾素（IFN）對人結(jié)腸癌細(xì)胞人類白細(xì)胞抗原DR(HLA-DR)表達(dá)的影響。
方法采用免疫組化SP法和ELISA法分別檢測不同劑量的α-IFN和γ-IFN刺激前、后HLA-DR在4株人結(jié)腸癌細(xì)胞HCT-8、Lovo、SW-480和Ls-174-T中的表達(dá)情況。
結(jié)果α-IFN和γ-IFN刺激之前，4株細(xì)胞中除Lovo細(xì)胞不表達(dá)HLA-DR，其他3株細(xì)胞均可不同程度表達(dá)HLA-DR; 刺激之后，4株細(xì)胞系的HLA-DR表達(dá)均有所增強(qiáng)，并呈現(xiàn)出IFN依賴性及γ-IFN的劑量依賴性，γ-IFN的誘導(dǎo)作用強(qiáng)于α-IFN。
結(jié)論IFN可以上調(diào)人結(jié)腸癌細(xì)胞HLA-DR的表達(dá)，IFN用于結(jié)腸癌的臨床治療具有一定的應(yīng)用前景。

引用本文： 黃文廣,劉鋼,方泉,黃漢菊. 干擾素對人結(jié)腸癌細(xì)胞HLA-DR表達(dá)的影響. 中國普外基礎(chǔ)與臨床雜志, 2005, 12(1): 15-18. doi: 復(fù)制

版權(quán)信息： ?四川大學(xué)華西醫(yī)院華西期刊社《中國普外基礎(chǔ)與臨床雜志》版權(quán)所有，未經(jīng)授權(quán)不得轉(zhuǎn)載、改編

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  1. 1. Mayer L, Eisenhardt D, Salomon P, et al. Expression of class II molecules on intestinal epithelial cells in humans. Differences between normal and inflammatory bowel disease [Ｊ]. Gastroenterology, 1991; 100(1)∶3.
  2. 2. Real FX, Carrato A, Schuessler MH, et al. IFNgammaregulated expression of a differentiation antigen of human cells [Ｊ]. J Immunol, 1988; 140(5)∶1571.
  3. 3. Lazaris AC, Theodoropoulos GE, Davaris PS, et al. Heat shock protein 70 and HLADR molecules tissue expression. Prognostic implications in colorectal cancer [Ｊ]. Dis Colon Rectum, 1995; 38(7)∶739.
  4. 4. Redondo M, RuizCabello F, Concha A, et al. Altered HLA class I expression in nonsmall cell lung cancer is independent of cmyc activation [Ｊ]. Cancer Res, 1991; 51(9)∶2463.
  5. 5. Gastl G, Marth C, Leiter E, et al. Effects of human recombinant alpha 2 arginterferon and gammainterferon on human breast cancer cell lines: dissociation of antiproliferative activity and induction of HLADR antigen expression [Ｊ]. Cancer Res, 1985; 45(7)∶2957.
  6. 6. Yang LJ, Sui YF, Chen ZN. Enhancement of HLADR expression in human hepatocellular carcinoma with interferon [Ｊ]. Chin J Cancer Res, 1999; 11(4)∶241.
  7. 7. Toes RE, Ossendorp F, Offringa R, et al. CD4 T cells and their role in antitumor immune responses [Ｊ]. J Exp Med, 1999; 189(5)∶753.