目的建立穩(wěn)定的大鼠部分肝移植動(dòng)脈化模型，并觀察術(shù)后病理變化。方法采用改良二袖套法建立大鼠部分肝移植模型，并進(jìn)行供體的腹腔動(dòng)脈與受體的右腎動(dòng)脈端端吻合，采用肝穿刺活檢技術(shù)于術(shù)后1 d、2 d、4 d及7 d行肝活檢。結(jié)果動(dòng)物術(shù)后1周存活率為 85.4%。病理組織學(xué)檢查術(shù)后第2天可見細(xì)胞核有絲分裂象，第4天偶見細(xì)胞核有絲分裂象，第4、7天可見雙核細(xì)胞。結(jié)論通過技術(shù)改進(jìn)，提高了模型建立的穩(wěn)定性。肝穿刺活檢技術(shù)是進(jìn)行大鼠肝移植術(shù)后相關(guān)問題研究的簡便、安全、可行的方法。肝動(dòng)脈的重建可能加速了移植肝的術(shù)后再生。
引用本文： 黃新立,張峰,王學(xué)浩,成峰,陸森,錢曉峰,夏永祥. 建立大鼠部分肝移植動(dòng)脈化模型的實(shí)驗(yàn)研究△. 中國普外基礎(chǔ)與臨床雜志, 2004, 11(3): 224-227. doi: 復(fù)制
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| 2. | Kamada N, Calne RY. Orthotopic liver transplantation in the rat. Technique using cuff for portal vein anastomosis and biliary drainage [Ｊ]. Transplantation, 1979; 28(1)∶47. |
| 3. | Gao W, Lemasters JJ, Thurman RG. Development of a new method for hepatic rearterialization in rat orthotopic liver transplantation. Reduction of liver injury and improvement of surgical outcome by arterialization [Ｊ]. Transplantation, 1993; 56(1)∶19. |
| 4. | Reck T, Steinbauer F, Steinbauer M, et al. Impact of arterialization on hepatic oxygen supply, tissue energy phosphates, and outcome after liver transplantation in the rat [Ｊ]. Transplantation, 1996; 62(5)∶582. |
| 5. | Howden B, Jablonski P, Grossman H, et al. The importance of the hepatic artery in rat liver transplantation [Ｊ]. Transplantation, 1989; 47(3)∶428. |
| 6. | Omura T, Ascher NL, Emond JC. Fiftypercent partial liver transplantation in the rat [Ｊ]. Transplantation, 1996; 62(2)∶292. |
| 7. | 錢曉峰， 王學(xué)浩， 張峰， 等. 大鼠減體積肝移植模型的技術(shù)改進(jìn)及肝再生的初步觀察 [Ｊ]. 南京醫(yī)科大學(xué)學(xué)報(bào)， 2003； 23（3）∶221. |
| 8. | 孫鵬，蔡端， 張群華，等. 非協(xié)調(diào)性異種肝移植動(dòng)物模型的建立： 動(dòng)脈化模型與靜脈化模型的比較 [Ｊ]. 中華肝膽外科雜志， 2001； 7（7）∶442. |
| 9. | Li J, Dahmen U, Dirsch O, et al. Modified sleeve anastomosis for reconstruction of the hepatic artery in rat liver transplantation [Ｊ]. Microsurgery, 2002; 22(2)∶62. |
| 10. | Knoop M, Bachmann S, Keck H, et al. Experience with cuff rearterialization in 600 orthotopic liver grafts in the rat [Ｊ]. Am J Surg, 1994; 167(3)∶360. |
- 1. Lee S, Charters AC, Chandler JG, et al. A technique for orthotopic liver transplantation in the rat [Ｊ]. Transplantation, 1973; 16(6)∶664.
- 2. Kamada N, Calne RY. Orthotopic liver transplantation in the rat. Technique using cuff for portal vein anastomosis and biliary drainage [Ｊ]. Transplantation, 1979; 28(1)∶47.
- 3. Gao W, Lemasters JJ, Thurman RG. Development of a new method for hepatic rearterialization in rat orthotopic liver transplantation. Reduction of liver injury and improvement of surgical outcome by arterialization [Ｊ]. Transplantation, 1993; 56(1)∶19.
- 4. Reck T, Steinbauer F, Steinbauer M, et al. Impact of arterialization on hepatic oxygen supply, tissue energy phosphates, and outcome after liver transplantation in the rat [Ｊ]. Transplantation, 1996; 62(5)∶582.
- 5. Howden B, Jablonski P, Grossman H, et al. The importance of the hepatic artery in rat liver transplantation [Ｊ]. Transplantation, 1989; 47(3)∶428.
- 6. Omura T, Ascher NL, Emond JC. Fiftypercent partial liver transplantation in the rat [Ｊ]. Transplantation, 1996; 62(2)∶292.
- 7. 錢曉峰， 王學(xué)浩， 張峰， 等. 大鼠減體積肝移植模型的技術(shù)改進(jìn)及肝再生的初步觀察 [Ｊ]. 南京醫(yī)科大學(xué)學(xué)報(bào)， 2003； 23（3）∶221.
- 8. 孫鵬，蔡端， 張群華，等. 非協(xié)調(diào)性異種肝移植動(dòng)物模型的建立： 動(dòng)脈化模型與靜脈化模型的比較 [Ｊ]. 中華肝膽外科雜志， 2001； 7（7）∶442.
- 9. Li J, Dahmen U, Dirsch O, et al. Modified sleeve anastomosis for reconstruction of the hepatic artery in rat liver transplantation [Ｊ]. Microsurgery, 2002; 22(2)∶62.
- 10. Knoop M, Bachmann S, Keck H, et al. Experience with cuff rearterialization in 600 orthotopic liver grafts in the rat [Ｊ]. Am J Surg, 1994; 167(3)∶360.