目的探討大鼠阻塞性黃疸小腸組織中細(xì)胞間粘附分子-1（ICAM-1）和腫瘤壞死因子-α（TNF-α）的表達(dá)及意義。方法選SD大鼠24只，用隨機(jī)排列表法均分成假手術(shù)組（SO組）和阻塞性黃疸組（BDL組），每組術(shù)后再隨機(jī)分設(shè)7 d和14 d兩個(gè)時(shí)相點(diǎn)。應(yīng)用免疫組化的方法觀察小腸組織中ICAM-1表達(dá)的變化，采用雙抗體夾心ELISA法測(cè)定TNF-α含量，同時(shí)檢測(cè)小腸組織中髓過(guò)氧化物酶（MPO）活性、小腸組織和血漿中二胺氧化酶（DAO）活性。結(jié)果膽總管結(jié)扎后7 d、14 d，ICAM-1表達(dá)主要在小腸粘膜上皮細(xì)胞，且表達(dá)逐漸增強(qiáng)（P＜0.05）； 小腸粘膜TNF-α的含量逐漸上升，分別為（14.25±1.01） ng/g和（23.83±1.43） ng/g（P＜0.01）； 小腸DAO的活性逐漸降低，分別為（1.70±0.36） U/mg和（1.22±0.41） U/mg（P＜0.01）； 血漿DAO活性逐漸升高，分別為（6.44±1.74） U/ml和（8.93±1.29） U/ml（P＜0.01）； MPO活性逐漸增高，分別為（2.85±1.22 ） U/mg和（4.93±1.37） U/mg（P＜0.01）。結(jié)論阻塞性黃疸時(shí)小腸組織的ICAM1表達(dá)顯著上調(diào)和小腸粘膜TNFα含量顯著升高, 參與了中性粒細(xì)胞介導(dǎo)的小腸粘膜損傷。
引用本文： 繆輝來(lái),林木生,陳明,陳念平,包仕廷. 大鼠阻塞性黃疸小腸組織中細(xì)胞間粘附分子-1和腫瘤壞死因子-α的表達(dá)及其意義. 中國(guó)普外基礎(chǔ)與臨床雜志, 2004, 11(4): 331-334. doi: 復(fù)制
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