肝癌中HSP90α的表達(dá)及意義孫水平1吳勝利2耿智敏2牛新捷2楊威2
【摘要】目的研究熱休克蛋白90α(HSP90α)在人肝細(xì)胞性肝癌(HCC)組織中的表達(dá),并探討其與腫瘤生物學(xué)行為及預(yù)后的關(guān)系。方法采用免疫組織化學(xué)方法檢測(cè)10例正常肝組織、40例HCC組織及其癌旁組織中HSP90α的表達(dá)。結(jié)果HSP90α在正常肝臟、癌旁組織及肝細(xì)胞性肝癌中的表達(dá)陽(yáng)性率分別為10.0%、52.5%及72.5%,在肝細(xì)胞性肝癌中的表達(dá)陽(yáng)性率明顯高于正常肝組織和癌旁組織(P<0.05)。HCC 的HSP90α陽(yáng)性表達(dá)與腫瘤的臨床分期、分化程度、累及漿膜狀況及淋巴結(jié)轉(zhuǎn)移狀況顯著相關(guān)(P<0.05); 而與腫瘤數(shù)目無(wú)關(guān)(P gt;0.05)。HSP90α陰性表達(dá)者的預(yù)后顯著優(yōu)于HSP90α陽(yáng)性表達(dá)者,術(shù)后平均無(wú)瘤生存期前者為38.6個(gè)月,后者為25.5個(gè)月(P<0.05)。結(jié)論HSP90α在肝細(xì)胞性肝癌中的表達(dá)明顯增強(qiáng),HSP90α表達(dá)與HCC的臨床分期、腫瘤分化程度、累及漿膜狀況及淋巴結(jié)轉(zhuǎn)移狀況有關(guān),可作為判斷預(yù)后的指標(biāo)之一。
引用本文: 孫水平,吳勝利,耿智敏,牛新捷,楊威. 肝細(xì)胞肝癌中HSP90α的表達(dá)及意義. 中國(guó)普外基礎(chǔ)與臨床雜志, 2003, 10(3): 243-245. doi: 復(fù)制
1. | Mizushima Y, Wang P, Jarrar D, et al. Preinduction of heat shock proteins protects cardiac and hepatic functions following trauma and hemorrhage [J]. Am J Physiol Regul Integr Comp Physiol,2000; 278(2)∶R352. |
2. | 華赟鵬,梁力建,黃潔夫. 熱休克蛋白與肝缺血再灌注損傷 [J]. 中國(guó)普外基礎(chǔ)與臨床雜志, 2000; 7(4)∶269. |
3. | Hartl FU. Molecular chaperones in cellular protein folding [J]. Nature, 1996; 381(6583)∶571. |
4. | Kapitanovic M. Mechanical ileus caused by strangulation of the small intestine in a left paraduodenal hernia and in a defect in the root of the mesentery [J]. Lijec Vjesn, 1989; 111(12)∶454. |
5. | Yano M, Naito Z, Tanaka S, et al. Expression and roles of heat shock proteins in human breast cancer [J]. Jpn J Cancer Res, 1996; 87(9)∶ 908. |
6. | Blagosklonny MV, Toretsky J, Bohen S, et al. Mutant conformation of p53 translated in vitro or in vivo requires functional HSP90 [J]. Proc Natl Acad Sci USA, 1996; 93(16)∶8379. |
7. | 黃常志,周純,趙玫,等. 反義熱休克蛋白90 β對(duì)HeLa細(xì)胞惡性表型和藥敏感性的影響 [J]. 中華腫瘤雜志, 2000; 22(1)∶14. |
8. | Jameel A, Skilton RA, Campbell TA, et al. Clinical and biological significance of HSP90α in human breast cancer [J]. Int J Cancer, 1992; 50(3)∶409. |
9. | Mileo AM, Fanuele M, Battaglia F, et al. Selective overexpression of mRNA coding for 90 kDa stress protein in human ovarian cancer [J]. Anticancer Res, 1990; 10(4)∶903. |
10. | Nanbu K, Konishi I, Komatsu T, et al. Expression of heat shock proteins HSP70 and HSP90 in endometrial carcinomas. Correlation with clinicopathology, sex steroid receptor status, and p53 protein expression [J]. Cancer, 1996; 77(2)∶330. |
- 1. Mizushima Y, Wang P, Jarrar D, et al. Preinduction of heat shock proteins protects cardiac and hepatic functions following trauma and hemorrhage [J]. Am J Physiol Regul Integr Comp Physiol,2000; 278(2)∶R352.
- 2. 華赟鵬,梁力建,黃潔夫. 熱休克蛋白與肝缺血再灌注損傷 [J]. 中國(guó)普外基礎(chǔ)與臨床雜志, 2000; 7(4)∶269.
- 3. Hartl FU. Molecular chaperones in cellular protein folding [J]. Nature, 1996; 381(6583)∶571.
- 4. Kapitanovic M. Mechanical ileus caused by strangulation of the small intestine in a left paraduodenal hernia and in a defect in the root of the mesentery [J]. Lijec Vjesn, 1989; 111(12)∶454.
- 5. Yano M, Naito Z, Tanaka S, et al. Expression and roles of heat shock proteins in human breast cancer [J]. Jpn J Cancer Res, 1996; 87(9)∶ 908.
- 6. Blagosklonny MV, Toretsky J, Bohen S, et al. Mutant conformation of p53 translated in vitro or in vivo requires functional HSP90 [J]. Proc Natl Acad Sci USA, 1996; 93(16)∶8379.
- 7. 黃常志,周純,趙玫,等. 反義熱休克蛋白90 β對(duì)HeLa細(xì)胞惡性表型和藥敏感性的影響 [J]. 中華腫瘤雜志, 2000; 22(1)∶14.
- 8. Jameel A, Skilton RA, Campbell TA, et al. Clinical and biological significance of HSP90α in human breast cancer [J]. Int J Cancer, 1992; 50(3)∶409.
- 9. Mileo AM, Fanuele M, Battaglia F, et al. Selective overexpression of mRNA coding for 90 kDa stress protein in human ovarian cancer [J]. Anticancer Res, 1990; 10(4)∶903.
- 10. Nanbu K, Konishi I, Komatsu T, et al. Expression of heat shock proteins HSP70 and HSP90 in endometrial carcinomas. Correlation with clinicopathology, sex steroid receptor status, and p53 protein expression [J]. Cancer, 1996; 77(2)∶330.