• 1.瀘州醫(yī)學(xué)院附屬醫(yī)院血管外科（瀘州646000）;;
  • 2.瀘州醫(yī)學(xué)院組織胚胎學(xué)教研室;

目的探討肝移植大鼠術(shù)后早期肝竇內(nèi)皮細(xì)胞功能的改變及前列腺素E1（PGE1）對其的保護(hù)作用。方法用“兩袖套法”建立大 鼠原位肝移植模型，其中A組為正常大鼠空白對照組，B組為正常大鼠供體肝移植手術(shù)對照組，C組為休克性大鼠供體肝移植實(shí)驗(yàn)對照 組，D組為PGE1保護(hù)的休克性大鼠供體肝移植實(shí)驗(yàn)組，每組各8只。肝移植各組（無特別說明均指受體）于術(shù)后6 h取血，檢測其血清 中的肝臟酶學(xué)（ALT、LDH）、丙二醛（MDA）和一氧化氮（NO），以及血漿內(nèi)皮素（ET）水平，并取肝組織作常規(guī)病理學(xué)檢查，比較 各組有無差異。結(jié)果各移植組供肝冷保存時(shí)間及無肝期均接近，分別為（2±0.5） h和（15±3） min；　B、D組術(shù)后6 h全部存活， 存活率100%； C組術(shù)后6 h存活5只，存活率62.5%。與C組比較，D組ALT、LDH、MDA、ET明顯降低（P＜0.05），NO明顯升高（P＜0.05 ）。C組肝臟病理學(xué)檢查發(fā)現(xiàn)有明顯的組織學(xué)損害，而B、D組肝臟組織結(jié)構(gòu)基本完好。結(jié)論肝移植可造成肝竇內(nèi)皮細(xì)胞明顯損害，血 清NO和血漿ET水平可較好地反映肝竇內(nèi)皮細(xì)胞的功能狀態(tài)。PGE1通過穩(wěn)定血流動力學(xué)和穩(wěn)定肝竇內(nèi)皮細(xì)胞質(zhì)膜，可減輕移植肝肝竇內(nèi) 皮細(xì)胞的損害。

引用本文： 田根東,何延政,張艷敏,何春水. 肝移植大鼠肝竇內(nèi)皮細(xì)胞的損傷及PGE1對其的保護(hù)作用. 中國普外基礎(chǔ)與臨床雜志, 2003, 10(4): 328-331. doi: 復(fù)制

1. 1Kamada N, Calne RY. Orthotopic liver transplantation in the rat. Technique using cuff for portal vein anastomosis and biliary drainage [Ｊ].Transplantation, 1979; 28(1)∶47.
2. 3 Menger MD, Richter S, Yamauchi J, et al. Role of microcirculation in hepatic ischemia/reperfusion injury [Ｊ]. Hepatogastroenterology, 1999; 46(Suppl 2)∶1452.
3. 4Imamura H, Brault A, Huet PM. Effects of extended cold preservation and transplantation on the rat liver microcirculation [Ｊ]. Hepatology, 1997; 25(3)∶664.
4. 5Mitsuoka H，Suzuki S，Sakaguchi T，et al．Contribution of endothelin1 to microcirculatory impairment in total hepatic ischemia and reperfusion injury [Ｊ]. Transplantation, 1999; 67(4)∶514.
5. 6SerracinoInglott F, Habib NA, Mathie RT. Hepatic ischemiareperfusion injury [Ｊ]. Am J Surg, 2001; 181(2)∶ 160.
6. 7 Itasaka H, Suehiro T, Wakiyama S, et al. The mechanism of hepatic graft protection against reperfusion injury by prostaglandin E1 [Ｊ]. Surg Today, 1999; 29(6)∶526.
7. 8Oishi M, Tanaka N, Orita K. Beneficial effects of prostaglandin E1 on hemodynamic changes during liver transplantation in pigs [Ｊ]. Transpl Int, 1996; 9(Suppl 1)∶S100.
8. 9季德剛，劉丙義，鄭澤霖，等．前列腺素E1對大鼠肝臟再灌注損傷的保護(hù)作用 [Ｊ].中國普外基礎(chǔ)與臨床雜志， 2003; 10(1)∶ 36.
9. 10Xu HS, Rosenlof LK, Pruett TL, et al. Prostaglandin E1 increases survival with extended anhepatic phase during liver transplantation [Ｊ]. Ann Surg, 1994; 220(1)∶53.
10. 11Kishimoto S, Sakon M, Umeshita K, et al. The inhibitory effect of prostaglandin E1 on oxidative stressinduced hepatocyte injury evaluated by calpainμ activation [Ｊ]. Transplantation, 2000; 69(11)∶2314.
11. 12Middleton A, Middleton B. Elevation of cyclic AMP by iloprost and prostaglandin E1 increases cholesterol efflux and the binding capacity for highdensity lipoproteins in human fibroblasts [Ｊ].Biochim Biophys Acta, 1998; 1391(2)∶117.
12. 嚴(yán)以群，楊甲梅，王軒，等．大鼠減體積肝移植模型的初步研究 [Ｊ].中華器官移植雜志， 1996； 17(2)∶55.
  1. 1. 1Kamada N, Calne RY. Orthotopic liver transplantation in the rat. Technique using cuff for portal vein anastomosis and biliary drainage [Ｊ].Transplantation, 1979; 28(1)∶47.
  2. 2. 3 Menger MD, Richter S, Yamauchi J, et al. Role of microcirculation in hepatic ischemia/reperfusion injury [Ｊ]. Hepatogastroenterology, 1999; 46(Suppl 2)∶1452.
  3. 3. 4Imamura H, Brault A, Huet PM. Effects of extended cold preservation and transplantation on the rat liver microcirculation [Ｊ]. Hepatology, 1997; 25(3)∶664.
  4. 4. 5Mitsuoka H，Suzuki S，Sakaguchi T，et al．Contribution of endothelin1 to microcirculatory impairment in total hepatic ischemia and reperfusion injury [Ｊ]. Transplantation, 1999; 67(4)∶514.
  5. 5. 6SerracinoInglott F, Habib NA, Mathie RT. Hepatic ischemiareperfusion injury [Ｊ]. Am J Surg, 2001; 181(2)∶ 160.
  6. 6. 7 Itasaka H, Suehiro T, Wakiyama S, et al. The mechanism of hepatic graft protection against reperfusion injury by prostaglandin E1 [Ｊ]. Surg Today, 1999; 29(6)∶526.
  7. 7. 8Oishi M, Tanaka N, Orita K. Beneficial effects of prostaglandin E1 on hemodynamic changes during liver transplantation in pigs [Ｊ]. Transpl Int, 1996; 9(Suppl 1)∶S100.
  8. 8. 9季德剛，劉丙義，鄭澤霖，等．前列腺素E1對大鼠肝臟再灌注損傷的保護(hù)作用 [Ｊ].中國普外基礎(chǔ)與臨床雜志， 2003; 10(1)∶ 36.
  9. 9. 10Xu HS, Rosenlof LK, Pruett TL, et al. Prostaglandin E1 increases survival with extended anhepatic phase during liver transplantation [Ｊ]. Ann Surg, 1994; 220(1)∶53.
  10. 10. 11Kishimoto S, Sakon M, Umeshita K, et al. The inhibitory effect of prostaglandin E1 on oxidative stressinduced hepatocyte injury evaluated by calpainμ activation [Ｊ]. Transplantation, 2000; 69(11)∶2314.
  11. 11. 12Middleton A, Middleton B. Elevation of cyclic AMP by iloprost and prostaglandin E1 increases cholesterol efflux and the binding capacity for highdensity lipoproteins in human fibroblasts [Ｊ].Biochim Biophys Acta, 1998; 1391(2)∶117.
  12. 12. 嚴(yán)以群，楊甲梅，王軒，等．大鼠減體積肝移植模型的初步研究 [Ｊ].中華器官移植雜志， 1996； 17(2)∶55.