• 江西醫(yī)學(xué)院第二附屬醫(yī)院普外一科（南昌330006）;

目的探討甘氨酸對大鼠肝臟熱缺血再灌注后肝竇內(nèi)皮細胞損傷的保護作用及其機理。方法取健康雄性SD大鼠72只，制備成鼠肝熱缺血再灌注模型,而后隨機分成正常對照組、缺血再灌注組、士的寧＋甘氨酸治療組和甘氨酸治療組, 每組各18只。分別于再灌注后1、3、24 h檢測血漿中內(nèi)皮素（ET）、透明質(zhì)酸（HA）、腫瘤壞死因子α （TNFα）、谷丙轉(zhuǎn)氨酶（ALT）及肝組織中超氧化物歧化酶（SOD）含量的變化，并在光鏡下觀察肝竇內(nèi)皮細胞的病理改變。結(jié)果在再灌注后的不同時點，甘氨酸治療組中ET、HA、TNFα及ALT含量較缺血再灌注組顯著降低(P＜0.01或P＜0.05），SOD值相應(yīng)升高，同時光鏡下肝竇內(nèi)皮細胞的病理變化明顯改善，士的寧可部分拮抗甘氨酸的作用。結(jié)論甘氨酸對鼠肝熱缺血再灌注后肝竇內(nèi)皮細胞的損傷具有保護作用。推測這種作用可能與肝竇內(nèi)皮細胞及枯否細胞膜上的甘氨酸受體密切相關(guān)。

引用本文： 舒明,熊本京,傅華群,蔣筱強,鄒書兵. 甘氨酸對鼠肝熱缺血再灌注后肝竇內(nèi)皮細胞損傷的保護作用. 中國普外基礎(chǔ)與臨床雜志, 2003, 10(4): 351-354. doi: 復(fù)制

版權(quán)信息： ?四川大學(xué)華西醫(yī)院華西期刊社《中國普外基礎(chǔ)與臨床雜志》版權(quán)所有，未經(jīng)授權(quán)不得轉(zhuǎn)載、改編

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8. Zhang Y, Ikejima K, Honda H, et al.Glycine prevents apoptosis of rat sinusoidal endothelial cells caused by deprivation of vascular endothelial growth factor [Ｊ]. Hepatology, 2000; 32(3)∶542.
9. Nishimura Y, Romer LH, Lemasters, et al. Mitochondrial dysfunction and cytoskeletal disruption during chemical hypoxia to cultured rat hepatic sinusoidal endothelial cells: the pH paradox and cytoprotection by glucose, acidotic pH, and glycine [Ｊ]. Hepatology, 1998; 27(4)∶1039.
  1. 1. Hayashi H, Chaudry IH, Clemens MG, et al. Hepatic ischemic modals for determining the effects of ATPMgCl2 treatment [Ｊ]. J Surg Res, 1986; 40(2)∶167.
  2. 2. 熊成龍，胡虹，陳孝平，等.常溫下肝缺血再灌注肝血竇內(nèi)皮細胞損傷 [Ｊ]. 中華外科雜志, 2000; 38(4)∶297.
  3. 3. Gujral JS, Bucci TJ, Farhood A, et al. Mechanism of cell death during warm hepatic ischemiareperfusion in rats: apoptosis or necrosis? [Ｊ]. Hepatology, 2001; 33(2)∶397.
  4. 4. Hisama N, Yamaguchi Y, Ishiko T, et al. Kupffer cell production of cytokineinduced neutrophil chemoattractant following ischemia/reperfusion injury in rats [Ｊ]. Hepatology, 1996; 24(5)∶1193.
  5. 5. Kourembanas S, Marsden PA, McQuillan LP, et al. Hypoxia induces endothelin gene expression and secretion in cultured human endothelium [Ｊ]. J Clin Invest, 1991; 88(3)∶1054.
  6. 6. Itasaka H, Kishikawa K, Suehiro T, et al. Serum hyaluronic acid for the assessment of graft viability in porcine liver transplantation [Ｊ]. Surg Today, 1994; 24(8)∶719.
  7. 7. Ikejima K, Qu W, Stachlewitz RF, et al. Kupffer cells contain a glycinegated chloride channel [Ｊ].Am J Physiol, 1997; 272(6 Pt 1)∶G1581.
  8. 8. Zhang Y, Ikejima K, Honda H, et al.Glycine prevents apoptosis of rat sinusoidal endothelial cells caused by deprivation of vascular endothelial growth factor [Ｊ]. Hepatology, 2000; 32(3)∶542.
  9. 9. Nishimura Y, Romer LH, Lemasters, et al. Mitochondrial dysfunction and cytoskeletal disruption during chemical hypoxia to cultured rat hepatic sinusoidal endothelial cells: the pH paradox and cytoprotection by glucose, acidotic pH, and glycine [Ｊ]. Hepatology, 1998; 27(4)∶1039.