目的探討重組人生長(zhǎng)激素（rhGH）在體外對(duì)人直腸癌細(xì)胞增殖的影響。方法實(shí)驗(yàn)分為對(duì)照組、rhGH組、奧沙利鉑（LOHP）組和rhGH+LOHP組4組,利用體外細(xì)胞培養(yǎng)、MTT比色技術(shù)及流式細(xì)胞儀等方法，測(cè)定不同濃度的rhGH對(duì)人直腸癌細(xì)胞株HR8348細(xì)胞倍增時(shí)間、細(xì)胞抑制率、細(xì)胞周期、增殖指數(shù)（PI）和DNA抑制率的影響。結(jié)果rhGH在體外不促進(jìn)HR8348細(xì)胞的分裂增殖，rhGH組與對(duì)照組比較，以及rhGH+LOHP組與LOHP組比較,其差異均無(wú)統(tǒng)計(jì)學(xué)意義（P gt;0.05）； rhGH+LOHP組與對(duì)照組比較及rhGH+LOHP組與對(duì)應(yīng)的rhGH組配對(duì)比較，細(xì)胞倍增時(shí)間明顯延長(zhǎng)，細(xì)胞抑制率增加，阻滯于G0~G1期的細(xì)胞數(shù)增加，S期和G2~M期細(xì)胞明顯減少，PI明顯降低，DNA抑制率顯著升高（P＜0.01，S期P＜0.05）。結(jié)論rhGH在體外不促進(jìn)直腸癌細(xì)胞的分裂增殖。
引用本文： 陳嘉勇,甘平,梁道明,譚晶,張毅. 重組人生長(zhǎng)激素在體外對(duì)人直腸癌細(xì)胞株HR8348增殖的影響. 中國(guó)普外基礎(chǔ)與臨床雜志, 2003, 10(4): 362-365. doi: 復(fù)制
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- 1. 祁小平，黎介壽，陳徹. 人生長(zhǎng)激素對(duì)癌細(xì)胞株周期動(dòng)力學(xué)的影響 [Ｊ]. 腸內(nèi)與腸外營(yíng)養(yǎng)， 2000; 7（2）∶83.
- 2. Estrov Z, Meir R, Barak Y, et al. Human growth hormone and insulinlike growth factor1 enhance the proliferation of human leukemic blasts [Ｊ]. J Clin Oncol, 1991; 9(3)∶394.
- 3. Raff T, Germann G. Growth hormone in surgeryan assessment of current knowledge [Ｊ]. Chirurg, 1997; 68(10)∶995.
- 4. 陳嘉勇，張捷，譚晶，等. 胃、 結(jié)腸癌患者術(shù)后生長(zhǎng)激素應(yīng)用的價(jià)值評(píng)估 [Ｊ]. 中國(guó)普外基礎(chǔ)與臨床雜志， 1999; 6（6）∶365.
- 5. OgilvyStuart AL, Shalet SM. Tumour occurrence and recurrence [Ｊ]. Horm Res, 1992; 38(Suppl 1)∶50.
- 6. Jauch KW, Heiss MM, Gruetzner U, et al. Prognostic significance of bone marrow micrometastases in patients with gastric cancer [Ｊ]. J Clin Oncol, 1996; 14(6)∶1810.
- 7. Heiss MM, Allgayer H, Gruetzner KU, et al. Individual development and uPAreceptor expression of disseminated tumour cells in bone marrow: a reference to early systemic disease in solid cancer [Ｊ]. Nat Med, 1995; 1(10)∶1035.
- 8. Fiebig HH, Dengler W, Hendriks HR. No evidence of tumor growth stimulation in human tumors in vitro following treatment with recombinant human growth hormone [Ｊ]. Anticancer Drugs, 2000; 11(8)∶659.
- 9. Bartlett DL, Stein TP, Torosian MH, et al. Effect of growth hormone and protein intake on tumor growth and host cachexia [Ｊ]. Surgery, 1995; 117(3)∶260.
- 10. Torosian MH，Donoway RB. Growth hormone inhibits tumors metastasis [Ｊ]. Cancer, 1991; 67(9)∶2280.
- 11. Wennbo H, GebreMedhin M, GritliLinde A, et al. Activation of the prolactin receptor but not the growth hormone receptor is important for induction of mammary tumors in transgenic mice [Ｊ]. J Clin Invest, 1997; 100(11)∶2744.