• 1.第二軍醫(yī)大學東方肝膽外科醫(yī)院（上海200438）;;
  • 2.昆明醫(yī)學院第二附屬醫(yī)院肝膽外科（昆明650101）;

目的探討鼠肝大部切除術后空、回腸粘膜固有層細胞免疫功能的變化及其與腸道細菌移位的關系。方法將48只SD大鼠隨機分為實驗組和假手術組，每組24只。實驗組切除70%肝臟，假手術組除不切除肝臟外，其余手術步驟同實驗組。分別于術后6、12、24和72 h取兩組大鼠（n=6）空、回腸粘膜冰凍切片，而后行免疫組化染色，觀察不同時相腸粘膜固有層CD3+、CD4+、CD8+ T淋巴細胞的數量及肝臟功能的變化。結果實驗組術后24 h和72 h，其腸粘膜固有層CD3+、CD4+和CD8+T淋巴細胞的數量較假手術組明顯下降（P＜0.05），而兩組大鼠術后不同時相的ALT及AST變化，實驗組明顯高于假手術組（P lt;0.05）。結論鼠肝大部切除（70%）術后24 h，腸粘膜固有層CD3+、CD4+和CD8+ T淋巴細胞數量明顯降低。這種細胞免疫功能降低所導致的腸粘膜屏障功能受損，可能是造成腸道細菌移位的原因之一。

引用本文： 楊之斌,張炳彥,朱洪,王炳煌,郭永章. 鼠肝大部切除術后腸粘膜細胞免疫功能的變化及其與腸道細菌移位的關系. 中國普外基礎與臨床雜志, 2003, 10(5): 469-470. doi: 復制

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  1. 1. Wang X, Andersson R， Soltesz V, et al. Bacterial translocation after major hepatectomy in patients and rats [Ｊ]. Arch Surg, 1992; 127(9)∶ 1101.
  2. 2. Osterberg J, Johnsson C, Gannedahl G, et al. Alterations in mucosal immune cell distribution in septic rats [Ｊ]. Shock, 1997; 7(3)∶ 182.
  3. 3. Emond J, CapronLaudereau M, Meriggi F， et al. Extent of hepatectomy in the rat: evaluation of basal condition and effect of therapy [Ｊ]. Eur Surg Res, 1989; 21(5)∶ 251.
  4. 4. Woodcock NP, Robertson J, Morgan DR, et al. Bacterial translocation and immunohistochemical measurement of gut immune function [Ｊ]. J Clin Pathol, 2001; 54(8)∶ 619.
  5. 5. Gautreaux MD, Deitch EA, Berg RD. T lymphocyte in the host defense against translocation from the gastrointestinal tract [Ｊ]. Infect immun, 1994; 62(7)∶ 2874.
  6. 6. Wang X, Andersson R, Soltesz V， et al. Effect of portal hypertension on bacterial translocation induced by major liver resection in rats [Ｊ]. Eur J Surg, 1993; 159(6-7)∶ 343.
  7. 7. Nishimura M, Fujiyama Y, Niwakawa M, et al. In vivo cytokine responses in gutassociated lymphoid tissue (GALT) and spleen following oral administration of staphylococcal enterotoxin B [Ｊ]. Immunol Lett, 2002; 81(1)∶ 77.