• 南京醫(yī)科大學第一附屬醫(yī)院肝膽外科(南京210029);

為觀察灌注壓力對肝移植物活性的影響，實驗選擇SD大鼠，經(jīng)門靜脈在不同壓力下灌注4℃的Collin’s液以保存肝臟，然后應(yīng)用高效液相色譜儀檢測各組的高能磷酸化合物，制備出肝細胞懸液后采用陽離子測定儀測定肝細胞內(nèi)游離鈣濃度，并同時觀察了肝臟的超微結(jié)構(gòu)變化及光鏡病理改變。結(jié)果發(fā)現(xiàn)：隨灌注壓力的升高，肝臟的能量代謝、肝細胞內(nèi)游離鈣濃度以及超微結(jié)構(gòu)均有明顯改變。本實驗結(jié)果提示：不適當?shù)墓嘧毫蓪е赂闻K移植物的能量代謝的變化及肝細胞的鈣超載，從而影響肝移植物的活力； 為了取得高質(zhì)量的供肝，應(yīng)采用低溫低壓灌注。

引用本文： 張浩,王學浩,張峰. 灌注壓力對肝移植物活性的影響. 中國普外基礎(chǔ)與臨床雜志, 1999, 6(3): 140-142. doi: 復制

版權(quán)信息： ?四川大學華西醫(yī)院華西期刊社《中國普外基礎(chǔ)與臨床雜志》版權(quán)所有，未經(jīng)授權(quán)不得轉(zhuǎn)載、改編

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  1. 1. Ursula R, Maren H, Werner L. Energydependent injury to cultured sinusoidal endothelial cells of the rat liver in UW solution. Transplantation, 1993; 55(3)∶469.
  2. 2. Kamiikke W, Burdelski M, Steinhoff G, et al. Adenine nucleotide methabolism and its relation to organ viability in human liver transplantation. Transplantation, 1988; 45(1)∶138.
  3. 3. Hidication R, Rose Innes C, Tyler M, et al. Energy charge as an indication of liver viability. Transplantation, 1992; 53(4)∶540.
  4. 4. Foegh ML, Khirabadi BS, Ramwell PW. Prolongation of rat cardiac allograft survival by Ca2＋ blockers. Transplantation, 1985; 40(2)∶211.
  5. 5. Kimura J, Nakajima Y, Omura T, et al. Warm ischemia impairs calcium metabolism in porcine grafted liver. Transplant Proc, 1994; 26(4)∶2272.
  6. 6. Nishikawa Y, Ukida M, Matsuo R. Ca2＋ influx initiates death of hepatocytes injured by activation of complement. Liver, 1994; 14(4)∶200.