為了解急性壞死性胰腺炎(acute necrotizing pancreatitis，ANP)時(shí)血漿內(nèi)皮素(endothelin，ET)的變化及其病理意義，進(jìn)行了前瞻性動(dòng)物實(shí)驗(yàn)。即將SD大鼠隨機(jī)分為3組： 急性壞死性胰腺炎組(ANP組，n＝24)，經(jīng)膽胰管注入5%?；悄懰徕c(STC，1 ml/kg)制造ANP模型； 假手術(shù)組(SO組，n＝24)和血小板激活因子拮抗劑BN50739組(BN組，n＝24)。測(cè)定血漿ET及血小板激活因子(platelet activating factor，PAF)水平和胰腺微循環(huán)血流量，并進(jìn)行胰腺組織病理學(xué)評(píng)分。結(jié)果： ANP組在誘發(fā)胰腺炎后胰腺微循環(huán)血流量立即大幅度下降，于3小時(shí)時(shí)有所回升，以后再持續(xù)下降。血漿ET、PAF水平及病理記分均顯著高于SO組。BN組血流顯著改善，血漿ET、PAF水平及病理記分顯著低于ANP組。結(jié)論： 在STC誘發(fā)的大鼠ANP發(fā)病之初，存在胰腺缺血再灌注(I/R)現(xiàn)象，導(dǎo)致內(nèi)皮細(xì)胞損傷，血漿ET及PAF增加。I/R損傷、PAF與ET互相作用，形成惡性循環(huán)，加重胰腺病變。
引用本文： 李璽,黎介壽,朱維銘,李寧. 急性壞死性胰腺炎大鼠血漿內(nèi)皮素的改變及其意義. 中國普外基礎(chǔ)與臨床雜志, 1999, 6(6): 331-333. doi: 復(fù)制
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- 1. Takakuwa T, Endo S, Nakae H, et al. Relationships between plasma levels of typeI phospholipase A2, PAFacetylhydrolase, leukotriene B4, complements, endothelin1, and thrombomodulin in patients with sepsis. Res Commun Chem Pathol Pharmacol, 1994; 84(3)∶271.
- 2. Bassi D, Kollias N, FernandezDel Castillo C, et al. Impairment of pancreatic microcirculation correlates with the sererity of acute experimental pancreatitis. J Am Coll Surg, 1994; 179(3)∶257.
- 3. McMillen MA, Sumpio BE. Endothelins: polyfunctional cytokines. J Am Coll Surg, 1995; 180(5)∶621.
- 4. Aho HJ, Koskensalo SML, Nevalainen TJ. Experimental pancreatitis in the rat. Sodium taurocholateinduced acute haemorrhagic pancreatitis. Scand J Gastroenterol, 1980; 15(4)∶411.
- 5. Pinckard RN, Farr RS, Hanahan DJ. Physicochemical and functional identity of rabbit plateletactivating factor(PAF) released in vivo during IgE anaphylaxis with PAF released in vitro from IgE sensitized basophils. J Immunol, 1979; 123(4)∶1847.
- 6. Schmidt J, Lewandrowski K, FernanderDel Castillo C, et al. Histopathologic correlates of serum amylase activity in acute experimental pancreatitis. Dig Dis Sci, 1992; 37(9)∶1426.
- 7. Kusterer K, Poschmann T, Friedemann A, et al. Arterial constriction, ischemiareperfusion, and leukocyte adherence in acute pancreatitis. Am J Physiol, 1993; 265(1Pt1)∶G165.
- 8. Hoffmann TF, Leiderer R, Waldner H, et al. Ischemia reperfusion of the pancrease: a new in vivo model of acute pancreatitis in rats. Res Exp Med Berl, 1995; 195(3)∶125.
- 9. Anderson BO, Bensard DD, Harken AH. The role of platelet activating factor and its antagonists in shock, sepsis and multiple organ failure. Surg Gynecol Obstet, 1991; 1972(5)∶415.
- 10. Mustafa SB, Gandht CR, Harvey SAK, et al. Endothelin stimulates plateletactivating factor synthesis by cultured rat kupffer cells. Hepatology, 1995; 21(2)∶545.