• 1. 南京軍區(qū)南京總醫(yī)院全軍普通外科研究所(南京210002)2. 華西醫(yī)科大學(xué)附屬第一醫(yī)院肝膽胰外科3. 軍事醫(yī)學(xué)科學(xué)院附屬醫(yī)院血液科;

為探討細(xì)菌在膽固醇結(jié)石發(fā)病機(jī)理中的作用，從30例膽汁細(xì)菌培養(yǎng)陰性的膽囊膽固醇結(jié)石中提取DNA，應(yīng)用套式聚合酶鏈反應(yīng)擴(kuò)增細(xì)菌16S rRNA基因片段作為有細(xì)菌存在的分子證據(jù)。結(jié)果顯示，26例膽固醇結(jié)石中有細(xì)菌DNA存在。提示細(xì)菌可能在膽固醇結(jié)石的形成中具有一定的作用。

引用本文： 伍曉汀,肖路加,黎介壽,李昕權(quán). 套式聚合酶鏈反應(yīng)擴(kuò)增膽固醇結(jié)石中細(xì)菌DNA及其臨床意義研究. 中國(guó)普外基礎(chǔ)與臨床雜志, 1998, 5(2): 67-69. doi: 復(fù)制

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7. Sung JY, Costerton JW, Shaffer EA. Defense system in the biliary tract against bacterial infection. Dig Dis Sci, 1992; 37(5)∶689.
8. 伍曉汀，肖路加. 細(xì)菌在膽石形成中的作用研究進(jìn)展. 國(guó)外醫(yī)學(xué)外科學(xué)分冊(cè)， 1996； 23(5)∶277.
9. Neiderhiser DH, Roth HP, Webster LT. Studies on the importance of lecithin for cholesterol solubilization in bile. Gastroenterology, 1990; 99(1)∶1798.
10. Soloway RD, Growther RS. Bacteria and cholesterol gallstones: molecular biology comes to gallstones pathogenesis. Gastroenterology, 1995; 108(3)∶934.
  1. 1. Holzbach RT, Busch N. Nucleation and growth of cholesterol crystals. Gastroenterol Clin North Am, 1991; 20(1)∶67.
  2. 2. Swidsinski A, Ludwig N, Pahlig H, et al. Molecular genetic evidence of bacterial colonization of cholesterol gallstones. Gastroenterology, 1995; 108(3)∶860.
  3. 3. 林萬(wàn)明主編. PCR技術(shù)操作和應(yīng)用指南. 北京：人民軍醫(yī)出版社， 1995∶231～232.
  4. 4. Goodhart GL, Levison ME, Trotman BW, et al. Pigment vs cholesterol lithiasis. Bacteriology of gallbladder stone, bile and tissue correlated with biliary lipid analysis. Am J Dig Dis, 1978; 23(10)∶877.
  5. 5. Aurelius E, Johansson B, Skoldenberg B, et al. Rapid diagnosis of herpes simplex encephalitis by nested polymerase chain reaction assay of cerebrospinal fluid. Lancet, 1991; 337(8735)∶189.
  6. 6. Schleifer KH, Ludwig W. Phylogenetic relationships among bacteria. In： Fernholm B, Bremer K, Jornval H, eds. The hierarchy of life. Amsterdam: Elsevier, 1989∶ 278～282.
  7. 7. Sung JY, Costerton JW, Shaffer EA. Defense system in the biliary tract against bacterial infection. Dig Dis Sci, 1992; 37(5)∶689.
  8. 8. 伍曉汀，肖路加. 細(xì)菌在膽石形成中的作用研究進(jìn)展. 國(guó)外醫(yī)學(xué)外科學(xué)分冊(cè)， 1996； 23(5)∶277.
  9. 9. Neiderhiser DH, Roth HP, Webster LT. Studies on the importance of lecithin for cholesterol solubilization in bile. Gastroenterology, 1990; 99(1)∶1798.
  10. 10. Soloway RD, Growther RS. Bacteria and cholesterol gallstones: molecular biology comes to gallstones pathogenesis. Gastroenterology, 1995; 108(3)∶934.