目的 探討IL-10 對小鼠巨噬細(xì)胞髓樣分化因子88( MyD88) / 核因子κB( NF-κB) 炎癥信號活化的影響。方法 將小鼠巨噬細(xì)胞Ana-1 分為脂多糖( LPS) 組和LPS + IL-10 組, 分別于0. 5、1及2 h 收集巨噬細(xì)胞和細(xì)胞培養(yǎng)上清液,Western blot 檢測細(xì)胞MyD88 與胞漿、胞核NF-κB p65 亞基表達(dá), ELISA 法檢測培養(yǎng)上清中腫瘤壞死因子α( TNF-α) 含量。結(jié)果 在0 ～2 h, LPS 組細(xì)胞MyD88表達(dá)顯著持續(xù)上升, LPS +IL-10 組于LPS刺激后上升, 0. 5 h 達(dá)峰值, 2 h 恢復(fù)至正常水平, 1 h 和2 h相對含量均低于LPS 組( 11. 6 ±1. 3 比17. 5 ±0. 7, 8. 8 ±0. 3 比21. 4 ±1. 8, P lt; 0. 05) ; 總NF-κB 表達(dá)量在兩組間無明顯差異。NF-κB 核漿比變化趨勢與MyD88 類似, LPS + IL-10 組1 h 及2 h 相對含量亦均低于LPS 組( 1. 1 ±0. 1 比2. 4 ±0. 4,0. 6 ±0. 7 比3. 1 ±0. 6, P lt;0. 05) ; 相應(yīng)的, LPS+ IL-10 組1 h和2 h TNF-α含量亦低于LPS 組[ ( 222. 5 ±33. 5) pg/mL 比( 365. 2 ±22. 7 ) pg/mL, ( 212. 7 ±15. 9) pg/mL比( 566. 2 ±31. 5) pg/mL, P lt;0. 05] 。結(jié)論 IL-10 通過抑制減少MyD88/NF-κB 信號通路活化, 降低TNF-α表達(dá), 從而下調(diào)炎癥反應(yīng)強度。
引用本文： 藍(lán)楊,李理,袁偉鋒,黃文杰. IL-10 對脂多糖誘導(dǎo)Ana-1 細(xì)胞的MyD88 /核因子κB 活性影響的研究. 中國呼吸與危重監(jiān)護(hù)雜志, 2010, 9(3): 243-245. doi: 復(fù)制
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- 1. Inoue G. Effect of interleukin-10 ( IL-10 ) on experimental LPSinduced acute lung injury. J Infect Chemother, 2000 , 6: 51 -60 .
- 2. 陳業(yè)民, 黃文杰, 李勝利, 等. 重癥肺炎大鼠干擾素-γ、白細(xì)胞介素-6 和腫瘤壞死因子-α含量變化. 中國病理生理雜志, 2007, 23 :492-494.
- 3. 李勝利, 黃文杰. 地塞米松對SD 大鼠肺炎克雷伯桿菌性重癥肺炎模型血清白細(xì)胞介素-8、10 的影響. 實用醫(yī)學(xué)雜志, 2007, 23 :2634-2635.
- 4. Reutershan J, Basit A, Galkina EV, et al. Sequential recruitment of neutrophils into lung and lung injury bronchoalveolar lavage fluid in LPS-induced acute lung injury. Am J Physiol Lung Cell Mol Physiol,2005, 289 : 807-815.
- 5. Wanidworanun C, Strober W. Predominant role of tumor necrosis factor-alpha in human monocyte IL-10 synthesis. J Immunol, 1993 ,151: 6853 -6861 .
- 6. Wang P, Wu P, Siegel MI, et al. Interleukin-10 inhibits nuclear factor kappa B( NF kappa B) activation in human monocytes. IL-10 and IL-4 suppress cytokine synthesis by different mechanisms. J Biol Chem,1995, 270 : 9558 -9563 .
- 7. Schottelius AJ, Mayo MW, Sartor RB, et al. Interleukin-10 signaling blocks inhibitor of kappa B kinase activity and nuclear factor-kappa B DNA binding. J Biol Chem, 1999 , 274: 31868 -31874.
- 8. Zhang FX, Kirschning CJ, Mancinelli R, et al. Lipopolysaccharide up regulates the expression of Toll-like receptor 4 in human vascular endothelial cells. Chin Med J( Engl) , 2002, 115: 286-289.