目的 通過建立煙曲霉菌絲感染人臍靜脈內(nèi)皮細(xì)胞( HUVEC) 的體外模型, 觀察煙曲霉菌絲感染后HUVEC 表達(dá)血管假性血友病因子( vWF) 的變化, 以及用不同干預(yù)因素處理對(duì)HUVEC表達(dá)vWF 的影響, 以探討侵襲性曲霉病血管侵襲的機(jī)制。方法 實(shí)驗(yàn)分六組, 即空白對(duì)照組、TNF-α組、煙曲霉菌絲組、細(xì)胞松弛素D組、N-鈣黏蛋白抗體組和地塞米松組, 分別于2、6、12 及18 h 提取細(xì)胞培養(yǎng)上清液, 采用ELISA 法檢測(cè)vWF濃度。結(jié)果 與2 h 比較, 空白對(duì)照組和TNF-α組的vWF表達(dá)量在18 h 增加( P lt;0. 05) , 其余各組vWF表達(dá)量在6、12 及18 h 均增加( P lt;0. 05) ; 各實(shí)驗(yàn)組與空白對(duì)照組比較, 除N-鈣黏蛋白抗體組HUVEC 的vWF表達(dá)量在2 h無明顯變化外, 其余各時(shí)間點(diǎn)vWF表達(dá)量均升高( P lt;0. 05) 。TNF-α組HUVEC 的vWF 表達(dá)量在2 h 高于煙曲霉菌絲組, 而在18 h 低于煙曲霉菌絲組。N-鈣黏蛋白抗體組HUVEC 的vWF 表達(dá)量在2 h 和6 h 分別低于煙曲霉菌絲組相應(yīng)時(shí)間點(diǎn)。細(xì)胞松弛素D 組和地塞米松組vWF 在各時(shí)間點(diǎn)與煙曲霉菌絲組的差異均無統(tǒng)計(jì)學(xué)意義。結(jié)論 煙曲霉菌絲感染后, HUVEC 過度表達(dá)vWF, N-鈣黏蛋白單克隆抗體可減少vWF 的表達(dá), 而細(xì)胞松弛素D 和地塞米松對(duì)vWF的表達(dá)無明顯影響。
引用本文： 申玉英,徐小勇,張峰,張鵬鵬,施毅. 煙曲霉感染對(duì)人臍靜脈內(nèi)皮細(xì)胞表達(dá)血管假性血友病因子的影響. 中國(guó)呼吸與危重監(jiān)護(hù)雜志, 2010, 9(5): 498-500. doi: 復(fù)制
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- 1. Barth PJ, Rossberg C, Koch S, et al. Pulmonary aspergillosis in an unselected autopsy series. Pathol Res Pract, 2000, 196: 73 -80 .
- 2. 榮令, 周新, 李峰, 等. 侵襲性肺曲霉病小鼠的凝血功能異常及低分子肝素的治療作用. 中國(guó)呼吸與危重監(jiān)護(hù)雜志,2008, 7: 440-444.
- 3. Lopes Bezerra LM, Filler SG. Interactions of Aspergillus fumigatus with endothelial cells: internalization, injury, and stimulation of tissue factor activity. Blood, 2004, 103: 2143-2149.
- 4. Ruggeri ZM, Ware J. The structure and function of von Willbrand factor. Thromb Haemost, 1992, 67 : 594-599.
- 5. Rondaij MG, Sellink E, Gijzen KA, et al. Small GTP-Binding Protein Ral Is Involved in cAMP-Mediated Release of von Willebrand FactorFrom Endothelial Cells. Arterioscler Thromb Vasc Biol, 2004 , 24 :1315-1320.
- 6. Kamai Y, Lossinsky AS, Liu H, et al. Polarized response of endothelial cells to invasion by Aspergillus fumigatus. Cell Microbiol,2009, 11: 170-182 .
- 7. Leila M, Lopes Bezerra LM, Filler SG. Interactions of Aspergillus fumigatus with endothelial cells: internalization, injury, and stimulation of tissue factor activity. Blood, 2004, 103: 2143 -2149.
- 8. Schorer AE, Moldow CF, Rick ME. Interleukin 1 or endotoxin increases the release of von Willebrand factor from human endothelial cells. Br J Haematol, 1987, 67: 193-197.
- 9. 冼健坤, 陳蒙華, 謝露, 等. 大劑量腎上腺素對(duì)血管內(nèi)皮細(xì)胞表達(dá)血管性血友病因子的影響. 廣西醫(yī)科大學(xué)學(xué)報(bào),2009, 26: 169-172.