• 1重慶醫(yī)科大學(xué)中醫(yī)藥學(xué)院（重慶，400016）;;
  • 2重慶醫(yī)科大學(xué)臨床檢驗(yàn)診斷學(xué)教育部重點(diǎn)實(shí)驗(yàn)室;;
  • 3恩施市婦幼保健醫(yī)院內(nèi)兒科;

【摘 要】 目的  研究按摩對(duì)兔股四頭肌損傷的肌組織病理修復(fù)、細(xì)胞骨架蛋白結(jié)蛋白（Desmin）和α-肌動(dòng)蛋白（α-Actin）表達(dá)的效應(yīng)差異，探討按摩促進(jìn)肌肉損傷修復(fù)的作用機(jī)制。 方法  健康成年雄性新西蘭大白兔27只，體重（2.0 ± 0.5）kg，隨機(jī)分為3組，正常對(duì)照組（A組，n=3）、自然恢復(fù)組（B組，n=12）和按摩組（C組，n=12）。A組實(shí)驗(yàn)動(dòng)物不作任何處理，B、C組實(shí)驗(yàn)動(dòng)物用自制打擊器制備兔右后肢股四頭肌損傷模型。C組于造模后第8天開(kāi)始按摩，按摩轉(zhuǎn)速3 000～3 100 r/min，按摩時(shí)間15 min，每天1次至處死取材為止；B組不予按摩。B、C組于傷后14 d及21 d各取6只實(shí)驗(yàn)動(dòng)物股四頭肌樣本，HE染色觀察組織病理變化，免疫組織化學(xué)染色及Western blot檢測(cè)Desmin、α-Actin表達(dá)，并與A組正常股四頭肌標(biāo)本進(jìn)行比較。 結(jié)果  B、C組實(shí)驗(yàn)動(dòng)物均存活至實(shí)驗(yàn)完成。A組肌纖維形態(tài)規(guī)整，排列整齊，無(wú)結(jié)締樣組織；B組股四頭肌標(biāo)本見(jiàn)明顯組織壞死，多數(shù)肌纖維斷裂、萎縮、排列紊亂；C組骨骼肌組織形態(tài)、肌萎縮較B組改善，受損部位可見(jiàn)新生肌纖維。免疫組織化學(xué)染色顯示各時(shí)間點(diǎn)B、C組Desmin、α-Actin表達(dá)均低于A組（P  lt; 0.05）；但C組Desmin、α-Actin表達(dá)均較B組增強(qiáng)（P  lt; 0.05），且21 d時(shí)明顯高于14 d（P  lt; 0.05）。Western blot檢測(cè)示A組Desmin、α-Actin高于B、C組（P  lt; 0.05）， B組兩種蛋白表達(dá)最低。 結(jié)論  按摩可促進(jìn)兔損傷股四頭肌的組織形態(tài)及細(xì)胞骨架恢復(fù)，可能通過(guò)上調(diào)Desmin、α-Actin表達(dá)促進(jìn)骨骼肌組織損傷修復(fù)。

引用本文： 侯懿烜 ,柳滿然,余敏,張萍,劉祖麗,唐成林. 按摩促進(jìn)兔股四頭肌損傷修復(fù)的體內(nèi)研究. 中國(guó)修復(fù)重建外科雜志, 2012, 26(3): 346-351. doi: 復(fù)制

1. 11 Muroya S, Ertbjerg P, Pomponio L, et al. Desmin and troponin T are degraded faster in type IIb muscle fibers than in type I fibers during postmortem aging of porcine muscle. Meat Sci, 2010, 86(3): 764-769.
2. Zainuddin Z, Newton M, Sacco P, et al. Effects of massage on delayed-onset muscle soreness, swelling, and recovery of muscle function. J Athl Train, 2005, 40(3): 174-180.
3. Lovering RM, O’Neill A, Muriel JM, et al. Physiology, structure, and susceptibility to injury of skeletal muscle in mice lacking keratin 19-based and desmin-based intermediate filaments. Am J Physiol Cell Physiol, 2011, 300(4): C803-813.
4. Martinez-Amat A, Marchal-Corrales JA, Rodriguez-Serrano F, et al. Role of alpha-actin in muscle damage of injured athletes in comparison with traditional markers. Br J Sports Med, 2007, 41(7): 442-446.
5. Capetanaki Y, Milner DJ, Weitzer G. Desmin in muscle formation and maintenance: knockouts and consequences. Cell Struct Funct, 1997, 22(1): 103-116.
6. Tonino P, Pappas CT, Hudson BD, et al. Reduced myofibrillar connectivity and increased Z-disk width in nebulin-deficient skeletal muscle. J Cell Sci, 2010,123(Pt 3): 384-391.
7. 李世成, 吳維, 楊則宜. 運(yùn)動(dòng)后骨骼肌微結(jié)構(gòu)的損傷及修復(fù)與蛋白質(zhì)補(bǔ)充. 中國(guó)運(yùn)動(dòng)醫(yī)學(xué)雜志, 2004, 23(2): 216-219.
8. Crameri RM, Aagaard P, Qvortrup K, et al. Myofibre damage in human skeletal muscle: effects of electrical stimulation versus voluntary contraction. J Physiol, 2007, 583(Pt 1): 365-380.
9. Lieber RL, Friden J. Morphologic and mechanical basis of delayed-onset muscle soreness. J Am Acad Orthop Surg, 2002, 10(1): 67-73.
10. 金其貫, 劉霞, 李淑艷, 等. 反復(fù)離心運(yùn)動(dòng)致骨骼肌超微結(jié)構(gòu)變化的實(shí)驗(yàn)研究. 西安體育學(xué)院學(xué)報(bào), 2010, 27(4): 453-456.
11. Martinez-Amat A, Boulaiz H, Prados J, et al. Release of alpha-actin into serum after skeletal muscle damage. Br J Sports Med, 2005, 39(11): 830-834.
12. 張宏, 嚴(yán)雋陶, 徐俊, 等. 骨骼肌纖維類型增齡性變化特征及推拿手法對(duì)老齡大鼠骨骼肌纖維的影響. 中國(guó)組織工程研究與臨床康復(fù), 2007, 11(36): 7149-7152.
13. 王艷國(guó), 嚴(yán)雋陶, 房敏, 等. 推拿對(duì)頸椎病患者頸肌疲勞的影響. 江蘇中醫(yī)藥, 2008, 40(8): 45-47.
14. 秦渭志, 黃明喜, 陳金昌, 等. 推拿治療軟組織損傷的機(jī)制研究. 按摩與導(dǎo)引, 2007, 23(7): 8-9.
15. 田惠林, 趙斌, 劉玉倩, 等. 定量按摩對(duì)肌肉損傷修復(fù)作用的形態(tài)學(xué)和生物力學(xué)研究. 河北師范大學(xué)學(xué)報(bào)：自然科學(xué)版, 2005, 29(2): 213-216.
16. 李軍, 魏東明, 文新, 等. 推拿對(duì)家兔佐劑性關(guān)節(jié)炎微循環(huán)和血液流變學(xué)的影響. 中醫(yī)藥學(xué)報(bào), 2010, 38(1): 37-39.
17. 侯懿烜, 柳滿然, 張萍, 等. 骨骼肌損傷修復(fù)的生理生化機(jī)制研究. 激光生物學(xué)報(bào), 2011, 20(5): 662-667.
18. Jimena I, Tasset I, López-Martos R, et al. Effects of magnetic stimulation on oxidative stress and skeletal muscle regeneration induced by mepivacaine in rat. Med Chem, 2009, 5(1): 44-49.
19. Bär H, Schopferer M, Sharma S, et al. Mutations in desmin’s carboxy-terminal “tail” domain severely modify filament and network mechanics. J Mol Biol, 2010, 397(5): 1188-1198.
20. Haubold KW, Allen DL, Capetanaki Y, et al. Loss of desmin leads to impaired voluntary wheel running and treadmill exercise performance. J Appl Physiol, 2003, 95(4): 1617-1622.
21. 王瑞元, 苑玉和, 田文秀, 等. 一次力竭性離心運(yùn)動(dòng)后大鼠骨骼肌α-actin代謝變化及針刺對(duì)其影響. 體育科學(xué), 2002, 22(4): 95-99.
22. Mahoney DJ, Safdar A, Parise G, et al. Gene expression profiling in human skeletal muscle during recovery from eccentric exercise. Am J Physiol Regul Integr Comp Physiol, 2008, 294(6): R1901-1910.
23. Negishi S, Li Y, Usas A, et al. The effect of relaxin treatment on skeletal muscle injuries. Am J Sports Med, 2005, 33(12): 1816-1824.
  1. 1. 11 Muroya S, Ertbjerg P, Pomponio L, et al. Desmin and troponin T are degraded faster in type IIb muscle fibers than in type I fibers during postmortem aging of porcine muscle. Meat Sci, 2010, 86(3): 764-769.
  2. 2. Zainuddin Z, Newton M, Sacco P, et al. Effects of massage on delayed-onset muscle soreness, swelling, and recovery of muscle function. J Athl Train, 2005, 40(3): 174-180.
  3. 3. Lovering RM, O’Neill A, Muriel JM, et al. Physiology, structure, and susceptibility to injury of skeletal muscle in mice lacking keratin 19-based and desmin-based intermediate filaments. Am J Physiol Cell Physiol, 2011, 300(4): C803-813.
  4. 4. Martinez-Amat A, Marchal-Corrales JA, Rodriguez-Serrano F, et al. Role of alpha-actin in muscle damage of injured athletes in comparison with traditional markers. Br J Sports Med, 2007, 41(7): 442-446.
  5. 5. Capetanaki Y, Milner DJ, Weitzer G. Desmin in muscle formation and maintenance: knockouts and consequences. Cell Struct Funct, 1997, 22(1): 103-116.
  6. 6. Tonino P, Pappas CT, Hudson BD, et al. Reduced myofibrillar connectivity and increased Z-disk width in nebulin-deficient skeletal muscle. J Cell Sci, 2010,123(Pt 3): 384-391.
  7. 7. 李世成, 吳維, 楊則宜. 運(yùn)動(dòng)后骨骼肌微結(jié)構(gòu)的損傷及修復(fù)與蛋白質(zhì)補(bǔ)充. 中國(guó)運(yùn)動(dòng)醫(yī)學(xué)雜志, 2004, 23(2): 216-219.
  8. 8. Crameri RM, Aagaard P, Qvortrup K, et al. Myofibre damage in human skeletal muscle: effects of electrical stimulation versus voluntary contraction. J Physiol, 2007, 583(Pt 1): 365-380.
  9. 9. Lieber RL, Friden J. Morphologic and mechanical basis of delayed-onset muscle soreness. J Am Acad Orthop Surg, 2002, 10(1): 67-73.
  10. 10. 金其貫, 劉霞, 李淑艷, 等. 反復(fù)離心運(yùn)動(dòng)致骨骼肌超微結(jié)構(gòu)變化的實(shí)驗(yàn)研究. 西安體育學(xué)院學(xué)報(bào), 2010, 27(4): 453-456.
  11. 11. Martinez-Amat A, Boulaiz H, Prados J, et al. Release of alpha-actin into serum after skeletal muscle damage. Br J Sports Med, 2005, 39(11): 830-834.
  12. 12. 張宏, 嚴(yán)雋陶, 徐俊, 等. 骨骼肌纖維類型增齡性變化特征及推拿手法對(duì)老齡大鼠骨骼肌纖維的影響. 中國(guó)組織工程研究與臨床康復(fù), 2007, 11(36): 7149-7152.
  13. 13. 王艷國(guó), 嚴(yán)雋陶, 房敏, 等. 推拿對(duì)頸椎病患者頸肌疲勞的影響. 江蘇中醫(yī)藥, 2008, 40(8): 45-47.
  14. 14. 秦渭志, 黃明喜, 陳金昌, 等. 推拿治療軟組織損傷的機(jī)制研究. 按摩與導(dǎo)引, 2007, 23(7): 8-9.
  15. 15. 田惠林, 趙斌, 劉玉倩, 等. 定量按摩對(duì)肌肉損傷修復(fù)作用的形態(tài)學(xué)和生物力學(xué)研究. 河北師范大學(xué)學(xué)報(bào)：自然科學(xué)版, 2005, 29(2): 213-216.
  16. 16. 李軍, 魏東明, 文新, 等. 推拿對(duì)家兔佐劑性關(guān)節(jié)炎微循環(huán)和血液流變學(xué)的影響. 中醫(yī)藥學(xué)報(bào), 2010, 38(1): 37-39.
  17. 17. 侯懿烜, 柳滿然, 張萍, 等. 骨骼肌損傷修復(fù)的生理生化機(jī)制研究. 激光生物學(xué)報(bào), 2011, 20(5): 662-667.
  18. 18. Jimena I, Tasset I, López-Martos R, et al. Effects of magnetic stimulation on oxidative stress and skeletal muscle regeneration induced by mepivacaine in rat. Med Chem, 2009, 5(1): 44-49.
  19. 19. Bär H, Schopferer M, Sharma S, et al. Mutations in desmin’s carboxy-terminal “tail” domain severely modify filament and network mechanics. J Mol Biol, 2010, 397(5): 1188-1198.
  20. 20. Haubold KW, Allen DL, Capetanaki Y, et al. Loss of desmin leads to impaired voluntary wheel running and treadmill exercise performance. J Appl Physiol, 2003, 95(4): 1617-1622.
  21. 21. 王瑞元, 苑玉和, 田文秀, 等. 一次力竭性離心運(yùn)動(dòng)后大鼠骨骼肌α-actin代謝變化及針刺對(duì)其影響. 體育科學(xué), 2002, 22(4): 95-99.
  22. 22. Mahoney DJ, Safdar A, Parise G, et al. Gene expression profiling in human skeletal muscle during recovery from eccentric exercise. Am J Physiol Regul Integr Comp Physiol, 2008, 294(6): R1901-1910.
  23. 23. Negishi S, Li Y, Usas A, et al. The effect of relaxin treatment on skeletal muscle injuries. Am J Sports Med, 2005, 33(12): 1816-1824.