• 1. 中國醫(yī)科大學附屬盛京醫(yī)院普外二科（遼寧沈陽 110004）;;
  • 2. 中國醫(yī)科大學附屬盛京醫(yī)院兒科（遼寧沈陽 110004）;

目的 　探討豚鼠膽囊膽固醇結石形成過程中c-kit及scf mRNA及蛋白的表達變化情況。
方法 　將20只豚鼠隨機分為2組，分別給予普通飼料及致石飼料，致石周期為6周。RT-PCR檢測膽囊組織中c-kit及scf mRNA的表達情況，Western blot檢測c-kit及scf蛋白的表達情況。
結果 　RT-PCR結果顯示，與對照組相比，致石組豚鼠膽囊c-kit mRNA （t=6.985， P＜0.01）和scf mRNA （t=6.028，P＜0.01）的表達量下降。Western blot結果顯示，與對照組相比，致石組豚鼠膽囊c-kit （t=10.256，P＜0.01）及scf蛋白（t=9.586，P＜0.01）表達量下降。
結論 　飲食誘導的豚鼠膽囊膽固醇結石形成過程中，豚鼠膽囊c-kit和scf mRNA及蛋白表達水平均下降。c-kit/scf通路抑制可能參與膽囊膽固醇結石的形成。

引用本文： 范瑩,吳碩東,付倍蓓,殷振華. 高膽固醇飲食豚鼠膽囊組織中c-kit和scf?mRNA及蛋白的表達變化研究△. 中國普外基礎與臨床雜志, 2012, 19(10): 1063-1068. doi: 復制

1. Wang HH， Portincasa P， Liu M， et al. Effect of gallbladder hypomotility on cholesterol crystallization and growth in CCK-deficient mice[J]. Biochim Biophys Acta， 2010， 1801(2)：138-146.
2. Torihashi S， Ward SM， Nishikawa S， et al. c-kit-dependentdevelopment of interstitial cells and electrical activity in the murinegastrointestinal tract[J]. Cell Tissue Res， 1995， 280(1)：97-111.
3. Thuneberg L. Interstitial cells of Cajal：intestinal pacemaker cells?[J]. Adv Anat Embryol Cell Biol， 1982， 71：1-130.
4. Mazzia C， Porcher C， Julé Y， et al. Ultrastructural study of relationships between c-kit immunoreactive interstitial cells and other cellular elements in the human colon[J]. Histochem Cell Biol， 2000， 113(5)：401-411.
5. Horiguchi K， Sanders KM， Ward SM. Enteric motor neurons form synaptic-like junctions with interstitial cells of Cajal in the canine gastric antrum[J]. Cell Tissue Res， 2003， 311(3)：299-313.
6. Wang XY， Sanders KM， Ward SM. Intimate relationshipbetween interstitial cells of Cajal and enteric nerves in the guinea-pig small intestine[J]. Cell Tissue Res， 1999， 295(2)：247-256.
7. Ward SM， Beckett EA， Wang X， et al. Interstitial cells ofCajal mediate cholinergic neurotransmission from enteric motorneurons[J]. J Neurosci， 2000， 20(4)：1393-1403.
8. Sanders KM， Ward SM. Interstitial cells of Cajal：a new perspe-ctive on smooth muscle function[J]. J Physiol， 2006， 576(Pt 3)：721-726.
9. Hinescu ME， Ardeleanu C， Gherghiceanu M， et al. Interstitial Cajal-like cells in human gallbladder[J]. J Mol Histol， 2007， 38(4)：275-284.
10. Lavoie B， Balemba OB， Nelson MT， et al. Morphological and physiological evidence for interstitial cell of Cajal-like cells in the guinea pig gallbladder[J]. J Physiol， 2007， 579(Pt 2)：487-501.
11. Sun X， Yu B， Xu L ，et al. Interstitial cells of Cajal in the murine gallbladder[J]. Scand J Gastroenterol， 2006， 41(10)：1218-1226.
12. Hu WM， Luo HS， Ding XW， et al. Expression of c-kit messenger ribonucleic acid and c-kit protein in the gallbladders in guinea pigs of high cholesterol diet[J]. Dig Dis Sci， 2009， 54(8)：1651-1655.
13. Shaffer EA. Review article：control of gall-bladder motor function[J]. Aliment Pharmacol Ther，2000， 14(Suppl 2)：2-8.
14. Taylor AM， Galli SJ， Coleman JW. Stem-cell factor， the kit ligand， induces direct degranulation of rat peritoneal mast cells in vitro and in vivo：dependence of the in vitro effect on period of culture and comparisons of stem-cell factor with other mast cell-activating agents[J]. Immunology，1995，86(3)：427-433.
15. Kitamura Y， Hirota S， Nishida T. A loss-of-function mutation of c-kit results in depletion of mast cells and interstitial cells of Cajal，while its gain-of-function mutation results in their oncogenesis[J]. Mutat Res， 2001， 477(1-2)：165-171.
16. Fan Y， Wu SD， Fu BB. Effect of intestinal transit on the formation of cholesterol gallstones in hamsters[J]. Hepatobiliary Pancreat Dis Int， 2007， 6(5)：513-515.
17. Xu D， Yu BP， Luo HS， et al. Control of gallbladder contractions by cholecystokinin through cholecystokinin-A receptors on gallbladder interstitial cells of Cajal[J]. World J Gastroenterol， 2008， 14(18)：2882-2887.
18. Torihashi S， Yokoi K， Nagaya H， et al. New monoclonal antibody (AIC) identifies interstitial cells of Cajal in the musculature of the mouse gastrointestinal tract[J]. Auton Neurosci， 2004， 113(1-2)：16-23.
19. Ward SM， Sanders KM. Physiology and pathophysiology of the interstitial cell of Cajal：from bench to bedside.Ⅰ. Functional development and plasticity of interstitial cells of Cajal networks[J]. Am J Physiol Gastrointest Liver Physiol， 2001， 281(3)：G602-G611.
20. Torihashi S， Nishi K， Tokutomi Y， et al. Blockade of kit signaling induces transdifferentiation of interstitial cells of Cajal to a smooth muscle phenotype[J]. Gastroenterology， 1999， 117(1)：140-148.
21. Albertí E， Mikkelsen HB， Wang XY， et al. Pacemaker activity and inhibitory neurotransmission in the colon of Ws/Ws mutant rats[J]. Am J Physiol Gastrointest Liver Physiol， 2007， 292(6)：G1499-G1510.
22. Lyford GL， He CL， Soffer E， et al. Pan-colonic decrease in interstitial cells of Cajal in patients with slow transit constipation[J]. Gut， 2002， 51(4)：496-501.
23. Battaglia E， Bassotti G， Bellone G， et al. Loss of interstitial cells of Cajal network in severe idiopathic gastroparesis[J]. World J Gastroenterol， 2006， 12(38)：6172-6177.
24. Porcher C， Baldo M， Henry M， et al. Deficiency of interstitial cells of Cajal in the small intestine of patients with Crohn’s disease[J]. Am J Gastroenterol， 2002， 97(1)：118-125.
25. Orlic D， Kajstura J， Chimenti S， et al. Mobilized bone marrow cells repair the infarcted heart， improving function and survival[J]. Proc Natl Acad Sci U S A， 2001， 98(18)：10344-10349.
26. Ward SM， Ordög T， Bayguinov JR， et al. Development of interstitial cells of Cajal and pacemaking in mice lacking enteric nerves[J]. Gastroenterology， 1999， 117(3)：584-594.
27. Ward SM， Burns AJ， Torihashi S， et al. Impaired development of interstitial cells and intestinal electrical rhythmicity in steel mutants[J]. Am J Physiol Cell Physiol， 1995， 269(6 Pt 1)：C1577-C1585.
28. Rich A， Miller SM， Gibbons SJ， et al. Local presentation of steel factor increases expression of c-kit immunoreactive interstitial cells of Cajal in culture[J]. Am J Physiol Gastrointest Liver Physiol， 2003， 284(2)：G313-G320.
29. Nakahara M， Isozaki K， Vanderwinden JM， et al. Dose-dependent and time-limited proliferation of cultured murine interstitial cells of Cajal in response to stem cell factor[J]. Life Sci， 2002， 70(20)：2367-2376.
  1. 1. Wang HH， Portincasa P， Liu M， et al. Effect of gallbladder hypomotility on cholesterol crystallization and growth in CCK-deficient mice[J]. Biochim Biophys Acta， 2010， 1801(2)：138-146.
  2. 2. Torihashi S， Ward SM， Nishikawa S， et al. c-kit-dependentdevelopment of interstitial cells and electrical activity in the murinegastrointestinal tract[J]. Cell Tissue Res， 1995， 280(1)：97-111.
  3. 3. Thuneberg L. Interstitial cells of Cajal：intestinal pacemaker cells?[J]. Adv Anat Embryol Cell Biol， 1982， 71：1-130.
  4. 4. Mazzia C， Porcher C， Julé Y， et al. Ultrastructural study of relationships between c-kit immunoreactive interstitial cells and other cellular elements in the human colon[J]. Histochem Cell Biol， 2000， 113(5)：401-411.
  5. 5. Horiguchi K， Sanders KM， Ward SM. Enteric motor neurons form synaptic-like junctions with interstitial cells of Cajal in the canine gastric antrum[J]. Cell Tissue Res， 2003， 311(3)：299-313.
  6. 6. Wang XY， Sanders KM， Ward SM. Intimate relationshipbetween interstitial cells of Cajal and enteric nerves in the guinea-pig small intestine[J]. Cell Tissue Res， 1999， 295(2)：247-256.
  7. 7. Ward SM， Beckett EA， Wang X， et al. Interstitial cells ofCajal mediate cholinergic neurotransmission from enteric motorneurons[J]. J Neurosci， 2000， 20(4)：1393-1403.
  8. 8. Sanders KM， Ward SM. Interstitial cells of Cajal：a new perspe-ctive on smooth muscle function[J]. J Physiol， 2006， 576(Pt 3)：721-726.
  9. 9. Hinescu ME， Ardeleanu C， Gherghiceanu M， et al. Interstitial Cajal-like cells in human gallbladder[J]. J Mol Histol， 2007， 38(4)：275-284.
  10. 10. Lavoie B， Balemba OB， Nelson MT， et al. Morphological and physiological evidence for interstitial cell of Cajal-like cells in the guinea pig gallbladder[J]. J Physiol， 2007， 579(Pt 2)：487-501.
  11. 11. Sun X， Yu B， Xu L ，et al. Interstitial cells of Cajal in the murine gallbladder[J]. Scand J Gastroenterol， 2006， 41(10)：1218-1226.
  12. 12. Hu WM， Luo HS， Ding XW， et al. Expression of c-kit messenger ribonucleic acid and c-kit protein in the gallbladders in guinea pigs of high cholesterol diet[J]. Dig Dis Sci， 2009， 54(8)：1651-1655.
  13. 13. Shaffer EA. Review article：control of gall-bladder motor function[J]. Aliment Pharmacol Ther，2000， 14(Suppl 2)：2-8.
  14. 14. Taylor AM， Galli SJ， Coleman JW. Stem-cell factor， the kit ligand， induces direct degranulation of rat peritoneal mast cells in vitro and in vivo：dependence of the in vitro effect on period of culture and comparisons of stem-cell factor with other mast cell-activating agents[J]. Immunology，1995，86(3)：427-433.
  15. 15. Kitamura Y， Hirota S， Nishida T. A loss-of-function mutation of c-kit results in depletion of mast cells and interstitial cells of Cajal，while its gain-of-function mutation results in their oncogenesis[J]. Mutat Res， 2001， 477(1-2)：165-171.
  16. 16. Fan Y， Wu SD， Fu BB. Effect of intestinal transit on the formation of cholesterol gallstones in hamsters[J]. Hepatobiliary Pancreat Dis Int， 2007， 6(5)：513-515.
  17. 17. Xu D， Yu BP， Luo HS， et al. Control of gallbladder contractions by cholecystokinin through cholecystokinin-A receptors on gallbladder interstitial cells of Cajal[J]. World J Gastroenterol， 2008， 14(18)：2882-2887.
  18. 18. Torihashi S， Yokoi K， Nagaya H， et al. New monoclonal antibody (AIC) identifies interstitial cells of Cajal in the musculature of the mouse gastrointestinal tract[J]. Auton Neurosci， 2004， 113(1-2)：16-23.
  19. 19. Ward SM， Sanders KM. Physiology and pathophysiology of the interstitial cell of Cajal：from bench to bedside.Ⅰ. Functional development and plasticity of interstitial cells of Cajal networks[J]. Am J Physiol Gastrointest Liver Physiol， 2001， 281(3)：G602-G611.
  20. 20. Torihashi S， Nishi K， Tokutomi Y， et al. Blockade of kit signaling induces transdifferentiation of interstitial cells of Cajal to a smooth muscle phenotype[J]. Gastroenterology， 1999， 117(1)：140-148.
  21. 21. Albertí E， Mikkelsen HB， Wang XY， et al. Pacemaker activity and inhibitory neurotransmission in the colon of Ws/Ws mutant rats[J]. Am J Physiol Gastrointest Liver Physiol， 2007， 292(6)：G1499-G1510.
  22. 22. Lyford GL， He CL， Soffer E， et al. Pan-colonic decrease in interstitial cells of Cajal in patients with slow transit constipation[J]. Gut， 2002， 51(4)：496-501.
  23. 23. Battaglia E， Bassotti G， Bellone G， et al. Loss of interstitial cells of Cajal network in severe idiopathic gastroparesis[J]. World J Gastroenterol， 2006， 12(38)：6172-6177.
  24. 24. Porcher C， Baldo M， Henry M， et al. Deficiency of interstitial cells of Cajal in the small intestine of patients with Crohn’s disease[J]. Am J Gastroenterol， 2002， 97(1)：118-125.
  25. 25. Orlic D， Kajstura J， Chimenti S， et al. Mobilized bone marrow cells repair the infarcted heart， improving function and survival[J]. Proc Natl Acad Sci U S A， 2001， 98(18)：10344-10349.
  26. 26. Ward SM， Ordög T， Bayguinov JR， et al. Development of interstitial cells of Cajal and pacemaking in mice lacking enteric nerves[J]. Gastroenterology， 1999， 117(3)：584-594.
  27. 27. Ward SM， Burns AJ， Torihashi S， et al. Impaired development of interstitial cells and intestinal electrical rhythmicity in steel mutants[J]. Am J Physiol Cell Physiol， 1995， 269(6 Pt 1)：C1577-C1585.
  28. 28. Rich A， Miller SM， Gibbons SJ， et al. Local presentation of steel factor increases expression of c-kit immunoreactive interstitial cells of Cajal in culture[J]. Am J Physiol Gastrointest Liver Physiol， 2003， 284(2)：G313-G320.
  29. 29. Nakahara M， Isozaki K， Vanderwinden JM， et al. Dose-dependent and time-limited proliferation of cultured murine interstitial cells of Cajal in response to stem cell factor[J]. Life Sci， 2002， 70(20)：2367-2376.