楊盛家 1 , 陳兵 1 , 佟鑄 1 , 張淑文 2 , 史小林 3 , 許晴 3 , 張斌 1 , 張建 1
  • 1.首都醫(yī)科大學(xué)宣武醫(yī)院血管外科(北京 100053);;
  • 2.首都醫(yī)科大學(xué)宣武醫(yī)院再生醫(yī)學(xué)實(shí)驗(yàn)室(北京 100053);;
  • 3.首都醫(yī)科大學(xué)解剖組織與胚胎教研室(北京 100034);

目的應(yīng)用激光多普勒血流探測儀(LDF)和激光多普勒血流成像儀(LDPI)監(jiān)測Lewis大鼠后肢急性缺血模型血流和血壓的動(dòng)態(tài)變化，探討大鼠后肢急性缺血后血流變化特點(diǎn)。方法切除大鼠左后肢股動(dòng)脈制備急性后肢缺血模型，于術(shù)后2、7、14、28及49 d對手術(shù)側(cè)和非手術(shù)側(cè)肢體采用LDF進(jìn)行血流、血壓檢測，于術(shù)后7 d采用LDPI進(jìn)行血流檢測。結(jié)果所有大鼠術(shù)后均成活，未發(fā)現(xiàn)后肢壞死; 在術(shù)后14 d內(nèi)手術(shù)側(cè)后肢平均分為2分，在49 d平均分為1分。大鼠手術(shù)側(cè)和非手術(shù)側(cè)肢體血流比值在術(shù)后2 d由術(shù)前的1上升至1.31±0.439(P=0.021),術(shù)后7 d和14 d分別為0.82±0.538和0.93±0.294，兩者比較差異無統(tǒng)計(jì)學(xué)意義(P=0.502)，但均明顯低于術(shù)后2 d的值(P=0.032和P=0.019); 術(shù)后28 d下降到最低點(diǎn)(0.41±1.970)，明顯低于術(shù)后2、7和14 d值(P=0.004、P=0.007和P=0.006); 在術(shù)后49 d手術(shù)側(cè)后肢血流恢復(fù)到接近術(shù)前值(0.98±0.093)，明顯低于術(shù)后2 d（P=0.010）而高于術(shù)后28 d值(P=0.005)，與術(shù)后7 d和14 d的差異無統(tǒng)計(jì)學(xué)意義(P=0.126和P=0.382)。大鼠手術(shù)側(cè)和非手術(shù)側(cè)肢體血壓比值術(shù)后2 d由術(shù)前的1明顯下降至0.47±0.375(P=0.031); 術(shù)后7 d繼續(xù)下降至0.44±0.118，與術(shù)后2 d比較差異無統(tǒng)計(jì)學(xué)意義(P=0.203); 術(shù)后14 d下降到最低點(diǎn)(0.35±0.115)，明顯低于術(shù)后2 d和7 d值(P=0.001和P=0.036); 術(shù)后28 d開始上升(0.54±0.146)，明顯高于術(shù)后14 d值(P=0.008)，但與術(shù)后2 d(P=0.493)和7 d(P=0.551)的差異無統(tǒng)計(jì)學(xué)意義; 術(shù)后49 d恢復(fù)接近術(shù)前值(0.97±0.094)，明顯高于術(shù)后2、7、14和28 d值(P=0.013、P=0.021、P=0.002和P=0.031)。結(jié)論切除大鼠后肢股動(dòng)脈及分支的方法制備后肢急性缺血模型，在術(shù)后14~28 d患肢缺血處于最嚴(yán)重階段。LDF和LDPI可以動(dòng)態(tài)監(jiān)測肢體血流及血壓的變化，對監(jiān)測大鼠后肢術(shù)后缺血的動(dòng)態(tài)演變過程有著重要的作用。

引用本文： 楊盛家,陳兵,佟鑄,張淑文,史小林,許晴,張斌,張建. 應(yīng)用激光多普勒血流儀對大鼠后肢缺血模型血流動(dòng)力學(xué)的觀察. 中國普外基礎(chǔ)與臨床雜志, 2011, 18(2): 137-141. doi: 復(fù)制

1. Lindsberg PJ, O’Neill JT, Paakkari IA, et al. Validation of laser Doppler flowmetry in measurement of spinal cord blood flow [J]. Am J Physiol, 1989, 257(2 Pt 2): H674H680.
2. 劉鍵, 李安之, 張光寅. 激光多普勒微循環(huán)血流計(jì) [J]. 中國生物工程學(xué)報(bào), 1983, 2(4): 250253.
3. Schabauer AM, Booke TW. Cutaneous laser Doppler flowmetry: application and findings [J]. Mayo Clin Proc, 1994, 69(6): 564574.
4. Zwagerman N, Sprague S, Davis MD, et al. Preischemic exercise preserves cerebral blood flow during reperfusion in stroke [J]. Neurol Res, 2010, 32(5): 523529.
5. Li Z, Tam EW, Lau RY, et al. Post pressure response of skin blood flowmotions in anesthetized rats with spinal cord injury [J]. Microvasc Res, 2009, 78(1): 2024.
6. Pettigrew LC, Kindy MS, Scheff S, et al. Focal cerebral ischemia in the TNFalphatransgenic rat [J]. J Neuroinflammation, 2008, 5: 47.
7. Newman JM, Dwyer RM, StPierre P, et al. Decreased microvascular vasomotion and myogenic response in rat skeletal muscle in association with acute insulin resistance [J]. J Physiol, 2009, 587(Pt 11): 25792588.
8. 吳勁松, 陳銜城. 激光多普勒血流測定法在局部腦血流量監(jiān)測中的應(yīng)用 [J]. 國外醫(yī)學(xué)腦血管疾病分冊, 1999, 7(3): 162164.
9. Rendell MS, Finnegan MF, Healy JC. The relationship of laserDoppler skin blood flow measurements to the cutaneous microvascular anatomy [J]. Microvas Res, 1998, 55(1): 313.
10. 楊劍, 劉博. 應(yīng)用Perimed激光多普勒血流儀對家兔耳蝸血流的測量 [J]. 中國醫(yī)學(xué)裝備, 2007, 4(12): 4042.
11. Line PD, Mowinicke P, Lien B, et al. Repeated measurement variation and precision of laser Doppler flowmetry measurements [J]. Microvas Res, 1992, 43(3): 285293.
12. 王寧, 陳興新, 陳磊, 等. 血流多普勒信號采集與顯示系統(tǒng)的設(shè)計(jì) [J]. 中國醫(yī)療器械信息, 2008, 14(10): 4749.
13. 邵洪波, 姚月明, 張慶富, 等. 己酮可可堿干預(yù)隨意皮瓣的成活激光多普勒血液灌流成像評價(jià) [J]. 中國組織工程研究與臨床康復(fù), 2010, 14(5): 838842.
14. Eichhorn W, Auer T, Voy ED. Diagnostic imaging of microcirculation of pedicled flapplasties of the mandibulofacial area using the laser Doppler scanner [J]. Handchir Mikrochir Plast Chir, 1995, 27(4): 176180.
15. Murray AK, Herrick AL, King TA. Laser Doppler imaging: a developing technique for application in the rheumatic diseases [J]. Rheumatology (Oxford), 2004, 43(10): 12101218.
16. Karamürsel S, Kayikioｇˇ lu A, Aksoy HM, et al. Dorsal visor flap in fingertip reconstruction [J]. Plast Reconstr Surg, 2001, 108(4): 10141018.
17. 黃曉元. 皮瓣移植在我國燒傷外科中的應(yīng)用 [J]. 中華燒傷雜志, 2008, 24(5): 352354.
18. 霍鑫, 馮金煒, 劉方峰, 等. pEGFPC1/Akt體外轉(zhuǎn)染骨髓間充質(zhì)干細(xì)胞對后肢缺血大鼠血管生成的影響 [J]. 中國普外基礎(chǔ)與臨床雜志, 2008, 15(6): 402407.
19. Pu LQ, Jackson S, Lachapelle KJ, et al. A persistent hindlimb ischemia model in the rabbit [J]. J Invest Surg, 1994, 7(1): 4960.
20. Couffinhal T, Silver M, Zheng LP, et al. Mouse model of angiogenesis [J]. Am J Pathol, 1998, 152(6): 16671679.
21. Ito WD, Arras M, Scholz D, et al. Angiogenesis but not collateral growth is associated with ischemia after femoral artery occlusion [J]. Am J Physiol, 1997, 273(3 Pt 2): H1255H1265.
22. 崔世軍, 俞恒錫, 滕旭, 等. 家兔后肢缺血模型的制作 [J]. 中國比較醫(yī)學(xué)雜志, 2003, 13(1): 3638.
23. 劉坤, 楊琨, 羅軍, 等. 結(jié)扎腹主動(dòng)脈加雙側(cè)腹壁陰部動(dòng)脈在大鼠后肢缺血模型中的應(yīng)用 [J]. 中國普外基礎(chǔ)與臨床雜志, 2010, 17(3): 258262.
  1. 1. Lindsberg PJ, O’Neill JT, Paakkari IA, et al. Validation of laser Doppler flowmetry in measurement of spinal cord blood flow [J]. Am J Physiol, 1989, 257(2 Pt 2): H674H680.
  2. 2. 劉鍵, 李安之, 張光寅. 激光多普勒微循環(huán)血流計(jì) [J]. 中國生物工程學(xué)報(bào), 1983, 2(4): 250253.
  3. 3. Schabauer AM, Booke TW. Cutaneous laser Doppler flowmetry: application and findings [J]. Mayo Clin Proc, 1994, 69(6): 564574.
  4. 4. Zwagerman N, Sprague S, Davis MD, et al. Preischemic exercise preserves cerebral blood flow during reperfusion in stroke [J]. Neurol Res, 2010, 32(5): 523529.
  5. 5. Li Z, Tam EW, Lau RY, et al. Post pressure response of skin blood flowmotions in anesthetized rats with spinal cord injury [J]. Microvasc Res, 2009, 78(1): 2024.
  6. 6. Pettigrew LC, Kindy MS, Scheff S, et al. Focal cerebral ischemia in the TNFalphatransgenic rat [J]. J Neuroinflammation, 2008, 5: 47.
  7. 7. Newman JM, Dwyer RM, StPierre P, et al. Decreased microvascular vasomotion and myogenic response in rat skeletal muscle in association with acute insulin resistance [J]. J Physiol, 2009, 587(Pt 11): 25792588.
  8. 8. 吳勁松, 陳銜城. 激光多普勒血流測定法在局部腦血流量監(jiān)測中的應(yīng)用 [J]. 國外醫(yī)學(xué)腦血管疾病分冊, 1999, 7(3): 162164.
  9. 9. Rendell MS, Finnegan MF, Healy JC. The relationship of laserDoppler skin blood flow measurements to the cutaneous microvascular anatomy [J]. Microvas Res, 1998, 55(1): 313.
  10. 10. 楊劍, 劉博. 應(yīng)用Perimed激光多普勒血流儀對家兔耳蝸血流的測量 [J]. 中國醫(yī)學(xué)裝備, 2007, 4(12): 4042.
  11. 11. Line PD, Mowinicke P, Lien B, et al. Repeated measurement variation and precision of laser Doppler flowmetry measurements [J]. Microvas Res, 1992, 43(3): 285293.
  12. 12. 王寧, 陳興新, 陳磊, 等. 血流多普勒信號采集與顯示系統(tǒng)的設(shè)計(jì) [J]. 中國醫(yī)療器械信息, 2008, 14(10): 4749.
  13. 13. 邵洪波, 姚月明, 張慶富, 等. 己酮可可堿干預(yù)隨意皮瓣的成活激光多普勒血液灌流成像評價(jià) [J]. 中國組織工程研究與臨床康復(fù), 2010, 14(5): 838842.
  14. 14. Eichhorn W, Auer T, Voy ED. Diagnostic imaging of microcirculation of pedicled flapplasties of the mandibulofacial area using the laser Doppler scanner [J]. Handchir Mikrochir Plast Chir, 1995, 27(4): 176180.
  15. 15. Murray AK, Herrick AL, King TA. Laser Doppler imaging: a developing technique for application in the rheumatic diseases [J]. Rheumatology (Oxford), 2004, 43(10): 12101218.
  16. 16. Karamürsel S, Kayikioｇˇ lu A, Aksoy HM, et al. Dorsal visor flap in fingertip reconstruction [J]. Plast Reconstr Surg, 2001, 108(4): 10141018.
  17. 17. 黃曉元. 皮瓣移植在我國燒傷外科中的應(yīng)用 [J]. 中華燒傷雜志, 2008, 24(5): 352354.
  18. 18. 霍鑫, 馮金煒, 劉方峰, 等. pEGFPC1/Akt體外轉(zhuǎn)染骨髓間充質(zhì)干細(xì)胞對后肢缺血大鼠血管生成的影響 [J]. 中國普外基礎(chǔ)與臨床雜志, 2008, 15(6): 402407.
  19. 19. Pu LQ, Jackson S, Lachapelle KJ, et al. A persistent hindlimb ischemia model in the rabbit [J]. J Invest Surg, 1994, 7(1): 4960.
  20. 20. Couffinhal T, Silver M, Zheng LP, et al. Mouse model of angiogenesis [J]. Am J Pathol, 1998, 152(6): 16671679.
  21. 21. Ito WD, Arras M, Scholz D, et al. Angiogenesis but not collateral growth is associated with ischemia after femoral artery occlusion [J]. Am J Physiol, 1997, 273(3 Pt 2): H1255H1265.
  22. 22. 崔世軍, 俞恒錫, 滕旭, 等. 家兔后肢缺血模型的制作 [J]. 中國比較醫(yī)學(xué)雜志, 2003, 13(1): 3638.
  23. 23. 劉坤, 楊琨, 羅軍, 等. 結(jié)扎腹主動(dòng)脈加雙側(cè)腹壁陰部動(dòng)脈在大鼠后肢缺血模型中的應(yīng)用 [J]. 中國普外基礎(chǔ)與臨床雜志, 2010, 17(3): 258262.