• 1.瀘州醫(yī)學(xué)院附屬醫(yī)院血管外科(四川瀘州646000);;
  • 2.江蘇省常州市第一人民醫(yī)院血管外科（常州213017）;;
  • 3.忠縣人民醫(yī)院肝膽外科(重慶忠縣404300);

目的 觀察內(nèi)皮祖細(xì)胞（EPC）在血管新生中的作用及其生物學(xué)特性。
方法 取大鼠外周血分離出EPC，觀察其在體外的培養(yǎng)和擴增情況，對培養(yǎng)的EPC進(jìn)行檢測，同時建立三維立體模型并分析。
結(jié)果 成功分離出外周血中的EPC，在平面培養(yǎng)時，各時間點經(jīng)VEGF誘導(dǎo)的EPC（實驗組）其增殖情況均優(yōu)于無VEGF誘導(dǎo)的EPC（對照組），P＜0.01。在由鼠尾膠原凝膠制成的三維基質(zhì)中EPC向膠原基質(zhì)內(nèi)生長，1 d內(nèi)即可出現(xiàn)向膠原內(nèi)的出芽及浸潤，并逐漸形成分支樣結(jié)構(gòu)； 實驗組生長快，向膠原基質(zhì)內(nèi)浸潤速度快、出芽快，管狀結(jié)構(gòu)粗大； 而對照組生長慢、出芽慢，管狀結(jié)構(gòu)細(xì)小，向膠原內(nèi)浸潤的深度淺，網(wǎng)狀結(jié)構(gòu)稀疏，不完整； 實驗組各時相三維基質(zhì)中新生血管數(shù)目均多于對照組（P＜0.01）。
結(jié)論 鼠尾膠原凝液可以誘導(dǎo)EPC參與血管新生的遷移、增殖、發(fā)芽等步驟； EPC三維基質(zhì)模型可用于新生血管的研究。VEGF能動員和誘導(dǎo)EPC促進(jìn)血管新生。

引用本文： 施森,何延政,劉勇,葛紅衛(wèi),胡波,文雪剛,曾宏,鐘武,楊輝. 外周血來源內(nèi)皮祖細(xì)胞三維血管新生模型的建立及其特性分析. 中國普外基礎(chǔ)與臨床雜志, 2009, 16(2): 119-123. doi: 復(fù)制

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11. Blacher S, Devy L, Burbridge MF, et al. Improved quantification of angiogenesis in the rat aortic ring assay [J]. Angiogenesis, 2001; 4(2)： 133.
12. Chalupowicz DG, Chowdhury ZA, Bach TL, et al. FibrinⅡ induces endothelial cell capillaary tube formation [J]. J Cell Biol, 1995; 130(1)： 207.
13. Wang HS, Hwang LL, Sue HF, et al. A simple quantitative method for evaluation of angiogenesis activity [J]. Assay Drug Dev Technol, 2004; 2(1)： 31.
  1. 1. 1Friedrich EB, Walenta K, Scharlau J, et al. CD34-/CD133+/VEGFR-2+ endothelial progenitor cell subpopulation with potent vasoregenerative capacities[J]. Circ Res, 2006; 98(3)： e20 2Kupatt C, Horstkotte J, Vlastos GA, et al. Embryonic endothelial progenitor cells expressing a broad range of proangiogenic and remodeling factors enhance vascularization and tissue recovery in acute and chronic ischemia[J]. FASEB J, 2005; 19(11)： 1576.
  2. 2. 8Montaez E, Casaroli-Marano RP, Vilaró S, et al. Comparative study of tube assembly in three-dimensional collagen matrix and on Matrigel coats [J]. Angiogenesis, 2002; 5(3)： 167 9Moiseeva EP, Fox LH, Howells LM, et al. Indole-3- carbinol-induced death in Cancer cells involves EGFR downregnlationand is exacerbated in a 3D environment [J]. Apoptosis, 2006; 11(5)： 799.
  3. 3. Kong D, Melo LG, Gnecchi M, et al. Cytokine-induced mobilization of circulating endothelial progenitor cells enhances repair of injured arteries [J].Circulation,2004; 110(14)： 2039.
  4. 4. Enomoto S, Yoshiyama M, Omura T, et al. Microbubble destruction with ultrasound augments neovascularisation by bone marrow cell transplantation in rat hind limb ischaemia[J]. Heart, 2006; 92(4)： 515.
  5. 5. Peichev M, Naiyer AJ, Pereira D, et al． Expression of VEGFR-2 and AC133 by circulating human CD34(+) cells identifies a population of functional endothelial precursors [J]. Blood, 2000; 95(3)： 952.
  6. 6. Shmelkov SV, Jun L, St Clair R, et al.Alternative promoters regulate transcription of the gene that encodes stem cell surface protein AC133 [J]. Blood, 2004; 103(6)： 2055.
  7. 7. 潘玉先,鄭振華. 人大隱靜脈內(nèi)皮細(xì)胞種植人工血管的實驗研究 [J]. 中國普外基礎(chǔ)與臨床雜志， 2000； 7（1）： 13.
  8. 8. Wang R, Xu J, Juliette L, et al. Three-dimensional co-culture models to study prostate cancer growth， progression， and metastasis to bone [J]. Semin Cancer Biol, 2005; 15(5)： 353.
  9. 9. Desoize B, Jardillier J. Multicellular resistance: a paradigm for clinical resistance[J]. Crit Rev Oneol Hematol, 2000; 36(2-3)： 193.
  10. 10. Ferrara N， Gerber HP, LeCouter J． The biology of VEGF and its receptors [J]. Nat Med, 2003; 9(6)： 669.
  11. 11. Blacher S, Devy L, Burbridge MF, et al. Improved quantification of angiogenesis in the rat aortic ring assay [J]. Angiogenesis, 2001; 4(2)： 133.
  12. 12. Chalupowicz DG, Chowdhury ZA, Bach TL, et al. FibrinⅡ induces endothelial cell capillaary tube formation [J]. J Cell Biol, 1995; 130(1)： 207.
  13. 13. Wang HS, Hwang LL, Sue HF, et al. A simple quantitative method for evaluation of angiogenesis activity [J]. Assay Drug Dev Technol, 2004; 2(1)： 31.