• 1.華中科技大學(xué)同濟(jì)醫(yī)學(xué)院附屬協(xié)和醫(yī)院普通外科(武漢 430022);;
  • 2.華中科技大學(xué)同濟(jì)醫(yī)學(xué)院附屬協(xié)和醫(yī)院普通外科研究室;;
  • 通訊作者： 陶凱雄;

目的檢測黑色素瘤抗原基因-3(MAGE-3)在直腸癌中的表達(dá),探索其與直腸癌臨床病理的關(guān)系及其在直腸癌免疫治療中的應(yīng)用價值。
方法采用RT-PCR法，對33例直腸癌患者的癌組織及其相應(yīng)距腫瘤邊緣（5±1） cm的癌旁組織和手術(shù)切緣（乙狀結(jié)腸端）以及3例直腸息肉標(biāo)本（無瘤）的MAGE-3的表達(dá)情況進(jìn)行檢測，并對RT-PCR擴增產(chǎn)物中的目的基因片段進(jìn)行DNA測序驗證。
結(jié)果33例直腸癌組織中，MAGE-3基因的表達(dá)陽性率為42.42%(14/33)，在癌旁組織和手術(shù)切緣組織中，MAGE-3基因的表達(dá)陽性率分別為18.18%(6/33)和15.15%(5/33)，3例直腸息肉標(biāo)本未見MAGE-3表達(dá)。腫瘤組織MAGE-3基因表達(dá)陽性率均顯著高于癌旁組織和手術(shù)切緣組織(P＜0.05)； 而與患者年齡、性別、腫瘤組織學(xué)類型、Dukes分期及淋巴結(jié)轉(zhuǎn)移無關(guān)(P gt;0.05)。
結(jié)論基于MAGE-3基因在直腸癌中的高表達(dá)率，MAGE-3表達(dá)的蛋白可以作為一種有前途的靶點用于免疫治療，同時有望成為一種篩查和隨訪指標(biāo)。

引用本文： 李新明,陶凱雄,王國斌,張寧,吳相柏,張景輝,田源. 黑色素瘤抗原基因-3在直腸癌的表達(dá)及意義. 中國普外基礎(chǔ)與臨床雜志, 2006, 13(3): 308-310下轉(zhuǎn)320. doi: 復(fù)制

版權(quán)信息： ?四川大學(xué)華西醫(yī)院華西期刊社《中國普外基礎(chǔ)與臨床雜志》版權(quán)所有，未經(jīng)授權(quán)不得轉(zhuǎn)載、改編

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