• 1.福建醫(yī)科大學(xué)附屬第二醫(yī)院普外科(泉州362000);;
  • 2.廈門大學(xué)抗癌研究中心(廈門361000);;
  • 3.福建醫(yī)科大學(xué)附屬協(xié)和醫(yī)院(福州350005);

目的  探討端粒酶檢測用于早期診斷原發(fā)性肝癌的可能性。
方法  采用TRAP法分別檢測22例原發(fā)性肝癌及癌旁組織標(biāo)本、12例肝硬變組織、6例腺瘤樣增生組織和10例正常肝組織中的端粒酶活性。
結(jié)果  22例原發(fā)性肝癌中有20例端粒酶表達(dá)陽性，陽性率為90.9%； 22例癌旁組織中有14例陽性，陽性率為63.6%； 12例肝硬變組織中有10例陽性，陽性率為83.3%； 6例腺瘤樣增生組織中5例陽性，陽性率為83.3%； 而10例正常肝組織的端粒酶檢測均為陰性。
結(jié)論  端粒酶活性表達(dá)可能發(fā)生在肝組織癌變過程中，并在其中扮演重要角色，因此端粒酶有可能成為肝癌早期診斷以及療效和預(yù)后判斷的一種標(biāo)志物。

引用本文： 王偉,楊善民,李新豐,黃長玉. 肝癌及其癌前病變組織端粒酶活性檢測的臨床意義探討. 中國普外基礎(chǔ)與臨床雜志, 2000, 7(4): 246-248. doi: 復(fù)制

1. 關(guān)曉飛， 孫志賢主編. 端粒酶活性檢測及其在惡性腫瘤診斷中的應(yīng)用 〔M〕. 北京： 軍事醫(yī)學(xué)科學(xué)出版社， 1992∶49～56.
2. Kim NW, Piatyszek MA, Prowse KR, et al. Specific association of human telomerase activity with immoratal cells and cancer 〔Ｊ〕. Science, 1994; 226(5193)∶2011.
3. Chadenean C, Hay K, Hirte HW, et al. Telomerase activity associated with acquistion of malignancy in human colorectal cancer 〔Ｊ〕. Cancer Res, 1995; 55(12)∶2573.
4. Counter CM, Cuptal J, Harley CB, et al. Telomerase activity in normal leukcoytes and in hematologic malignancies 〔Ｊ〕. Blood, 1995; 85(9)∶2315.
5. Tsujiuchi T, Tustsumi M, Kido A, et al. Increase telomerase activity in hyperplastic nodules and hepatocellular carcinomas induced by a cholinedeficient Lamino aciddefined diet in rats 〔Ｊ〕. Japan J Cancer Research, 1996; 87(11)∶1111.
6. Ohnishi M, Univ G, Med S, et al. Inhibitory effects of dietary protocatechuic acid and costunolide on 7, 12dimethybenz [a] anthraceneinduced hamster cheek pouch carcinogenesis 〔Ｊ〕. Japan J Cancer Research, 1997; 88(2)∶111.
7. Takara H, Nakanish T, Kitamoto M, et al. Telomerase activity in human liver tissues; comparison between chronic liver disease and hepatocellular carcinoma 〔Ｊ〕. Cancer Ris, 1995; 55(13)∶2734.
8. Kojima H, Yokosuka F, Imazeki F, et al. Telomerase activity and telomere length in hepatocellular carcinoma and chronic liver disease 〔Ｊ〕. Gastroenterology, 1997; 112(2)∶493.
9. Nakashiv K, Kitamoto M, Takara H, et al. Significance of telomerase activity of small differentiated hepatocellular carcinoma 〔Ｊ〕. Int J Cancer, 1997; 74(2)∶141.
10. Miura N, Horikawa I, Nashimoto H, et al. Progressive telomerase shortening and reactivation during hepatocellular carcinogenesis 〔Ｊ〕. Cancer Genet Cytogent, 1997; 93(1)∶56.
11. Bednarek A, Budunova I, Slaga TJ, et al. Increased telomerase activity in mouse skin premalignant progression 〔Ｊ〕. Cancer Res, 1995; 55(2)∶4566.
  1. 1. 關(guān)曉飛， 孫志賢主編. 端粒酶活性檢測及其在惡性腫瘤診斷中的應(yīng)用 〔M〕. 北京： 軍事醫(yī)學(xué)科學(xué)出版社， 1992∶49～56.
  2. 2. Kim NW, Piatyszek MA, Prowse KR, et al. Specific association of human telomerase activity with immoratal cells and cancer 〔Ｊ〕. Science, 1994; 226(5193)∶2011.
  3. 3. Chadenean C, Hay K, Hirte HW, et al. Telomerase activity associated with acquistion of malignancy in human colorectal cancer 〔Ｊ〕. Cancer Res, 1995; 55(12)∶2573.
  4. 4. Counter CM, Cuptal J, Harley CB, et al. Telomerase activity in normal leukcoytes and in hematologic malignancies 〔Ｊ〕. Blood, 1995; 85(9)∶2315.
  5. 5. Tsujiuchi T, Tustsumi M, Kido A, et al. Increase telomerase activity in hyperplastic nodules and hepatocellular carcinomas induced by a cholinedeficient Lamino aciddefined diet in rats 〔Ｊ〕. Japan J Cancer Research, 1996; 87(11)∶1111.
  6. 6. Ohnishi M, Univ G, Med S, et al. Inhibitory effects of dietary protocatechuic acid and costunolide on 7, 12dimethybenz [a] anthraceneinduced hamster cheek pouch carcinogenesis 〔Ｊ〕. Japan J Cancer Research, 1997; 88(2)∶111.
  7. 7. Takara H, Nakanish T, Kitamoto M, et al. Telomerase activity in human liver tissues; comparison between chronic liver disease and hepatocellular carcinoma 〔Ｊ〕. Cancer Ris, 1995; 55(13)∶2734.
  8. 8. Kojima H, Yokosuka F, Imazeki F, et al. Telomerase activity and telomere length in hepatocellular carcinoma and chronic liver disease 〔Ｊ〕. Gastroenterology, 1997; 112(2)∶493.
  9. 9. Nakashiv K, Kitamoto M, Takara H, et al. Significance of telomerase activity of small differentiated hepatocellular carcinoma 〔Ｊ〕. Int J Cancer, 1997; 74(2)∶141.
  10. 10. Miura N, Horikawa I, Nashimoto H, et al. Progressive telomerase shortening and reactivation during hepatocellular carcinogenesis 〔Ｊ〕. Cancer Genet Cytogent, 1997; 93(1)∶56.
  11. 11. Bednarek A, Budunova I, Slaga TJ, et al. Increased telomerase activity in mouse skin premalignant progression 〔Ｊ〕. Cancer Res, 1995; 55(2)∶4566.